נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Identification of BFN1, a bifunctional nuclease induced during leaf and stem senescence in Arabidopsis
Year:
2000
Source of publication :
Plant physiology (source)
Authors :
Lers, Amnon
;
.
Volume :
122
Co-Authors:
Perez-Amador, M.A., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Abler, M.L., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
De Rocher, E.J., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Thompson, D.M., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Van Hoof, A., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
LeBrasseur, N.D., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Lers, A., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Green, P.J., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Facilitators :
From page:
169
To page:
179
(
Total pages:
11
)
Abstract:
Nuclease I enzymes are responsible for the degradation of RNA and single-stranded DNA during several plant growth and developmental processes, including senescence. However, in the case of senescence the corresponding genes have not been reported. We describe the identification and characterization of BFN1 of Arabidopsis, and demonstrate that it is a senescence-associated nuclease I gene. BFN1 nuclease shows high similarity to the sequence of a barley nuclease induced during germination and a zinnia (Zinnia elegans) nuclease induced during xylogenesis. In transgenic plants overexpressing the BFN1 cDNA, a nuclease activity of about 38 kD was detected on both RNase and DNase activity gels. Levels of BFN1 mRNA were extremely low or undetectable in roots, leaves, and stems. In contrast, relatively high BFN1 mRNA levels were detected in flowers and during leaf and stem senescence. BFN1 nuclease activity was also induced during leaf and stem senescence. The strong response of the BFN1 gene to senescence indicated that it would be an excellent tool with which to study the mechanisms of senescence induction, as well as the role of the BFN1 enzyme in senescence using reverse genetic approaches in Arabidopsis.
Note:
Related Files :
arabidopsis
Asteraceae
Base Sequence
gene overexpression
germination
Ribonucleases
senescence
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24741
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:09
You may also be interested in
Scientific Publication
Identification of BFN1, a bifunctional nuclease induced during leaf and stem senescence in Arabidopsis
122
Perez-Amador, M.A., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Abler, M.L., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
De Rocher, E.J., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Thompson, D.M., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Van Hoof, A., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
LeBrasseur, N.D., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Lers, A., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Green, P.J., Dept. of Energy-Plant Research Lab., Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1312, United States
Identification of BFN1, a bifunctional nuclease induced during leaf and stem senescence in Arabidopsis
Nuclease I enzymes are responsible for the degradation of RNA and single-stranded DNA during several plant growth and developmental processes, including senescence. However, in the case of senescence the corresponding genes have not been reported. We describe the identification and characterization of BFN1 of Arabidopsis, and demonstrate that it is a senescence-associated nuclease I gene. BFN1 nuclease shows high similarity to the sequence of a barley nuclease induced during germination and a zinnia (Zinnia elegans) nuclease induced during xylogenesis. In transgenic plants overexpressing the BFN1 cDNA, a nuclease activity of about 38 kD was detected on both RNase and DNase activity gels. Levels of BFN1 mRNA were extremely low or undetectable in roots, leaves, and stems. In contrast, relatively high BFN1 mRNA levels were detected in flowers and during leaf and stem senescence. BFN1 nuclease activity was also induced during leaf and stem senescence. The strong response of the BFN1 gene to senescence indicated that it would be an excellent tool with which to study the mechanisms of senescence induction, as well as the role of the BFN1 enzyme in senescence using reverse genetic approaches in Arabidopsis.
Scientific Publication
You may also be interested in