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Citrus cell culture: Isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos
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Year:
1975
Source of publication :
Plant Science Letters
Authors :
ורדי, עליזה
;
.
שפיגל-רואי, פנחס
;
.
Volume :
4
Co-Authors:
Galun, E., The Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
231
To page:
236
(
Total pages:
6
)
Link :
Citrus cell culture: Isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos
Abstract:
Protoplasts have been isolated from non-differentiating ovular callus of Citrus sinensis and cultured in solid medium. Optimal plating density for cell division and colony formation was in the range of 105 cells per ml. Colony formation took place in about 4-9% of the protoplasts. Survival of protoplasts was reduced to about one half after exposure to 0.3% ethyl methanesulphonate (EMS) for one hour. Exposure of protoplasts to X-ray radiation resulted in half-survival at about 3400 R. Embryoid regeneration was promoted in callus from X-irradiated protoplasts. © 1975.
Note:
Related Files :
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More details
DOI :
10.1016/0304-4211(75)90280-1
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24743
Last updated date:
02/03/2022 15:27
Creation date:
16/04/2018 21:09
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תנאי שימוש
Citrus cell culture: Isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos
4
Galun, E., The Weizmann Institute of Science, Rehovot, Israel
Citrus cell culture: Isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos
Protoplasts have been isolated from non-differentiating ovular callus of Citrus sinensis and cultured in solid medium. Optimal plating density for cell division and colony formation was in the range of 105 cells per ml. Colony formation took place in about 4-9% of the protoplasts. Survival of protoplasts was reduced to about one half after exposure to 0.3% ethyl methanesulphonate (EMS) for one hour. Exposure of protoplasts to X-ray radiation resulted in half-survival at about 3400 R. Embryoid regeneration was promoted in callus from X-irradiated protoplasts. © 1975.
Scientific Publication