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The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types
Year:
2016
Source of publication :
Physiological Reports
Authors :
Yahav, Shlomo
;
.
Volume :
4
Co-Authors:
Harding, R.L., Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, United States
Halevy, O., Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
Yahav, S., Institute of Animal Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Velleman, S.G., Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, United States
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Skeletal muscle satellite cells are a muscle stem cell population that mediate posthatch muscle growth and repair. Satellite cells respond differentially to environmental stimuli based upon their fiber-type of origin. The objective of this study was to determine how temperatures below and above the in vitro control of 38°C affected the proliferation and differentiation of satellite cells isolated from the chicken anaerobic pectoralis major (p. major) or mixed fiber biceps femoris (b.femoris) muscles. The satellite cells isolated from the p. major muscle were more sensitive to both cold and hot temperatures compared to the b.femoris satellite cells during both proliferation and differentiation. The expressions of myogenic regulatory transcription factors were also different between satellite cells from different fiber types. MyoD expression, which partially regulates proliferation, was generally expressed at higher levels in p. major satellite cells compared to the b.femoris satellite cells from 33 to 43°C during proliferation and differentiation. Similarly, myogenin expression, which is required for differentiation, was also expressed at higher levels in p. major satellite cells in response to both cold and hot temperatures during proliferation and differentiation than b. femoris satellite cells. These data demonstrate that satellite cells from the anaerobic p. major muscle are more sensitive than satellite cells from the aerobic b. femoris muscle to both hot and cold thermal stress during myogenic proliferation and differentiation. © 2016 Published by the American Physiological Society and The Physiological Society.
Note:
Related Files :
Animal
Animals
Cell Proliferation
metabolism
myogenic factor
RT-PCR (Real-Time Polymerase Chain Reaction)
temperature
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More details
DOI :
10.14814/phy2.12770
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24777
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:10
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Scientific Publication
The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types
4
Harding, R.L., Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, United States
Halevy, O., Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
Yahav, S., Institute of Animal Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Velleman, S.G., Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, United States
The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types
Skeletal muscle satellite cells are a muscle stem cell population that mediate posthatch muscle growth and repair. Satellite cells respond differentially to environmental stimuli based upon their fiber-type of origin. The objective of this study was to determine how temperatures below and above the in vitro control of 38°C affected the proliferation and differentiation of satellite cells isolated from the chicken anaerobic pectoralis major (p. major) or mixed fiber biceps femoris (b.femoris) muscles. The satellite cells isolated from the p. major muscle were more sensitive to both cold and hot temperatures compared to the b.femoris satellite cells during both proliferation and differentiation. The expressions of myogenic regulatory transcription factors were also different between satellite cells from different fiber types. MyoD expression, which partially regulates proliferation, was generally expressed at higher levels in p. major satellite cells compared to the b.femoris satellite cells from 33 to 43°C during proliferation and differentiation. Similarly, myogenin expression, which is required for differentiation, was also expressed at higher levels in p. major satellite cells in response to both cold and hot temperatures during proliferation and differentiation than b. femoris satellite cells. These data demonstrate that satellite cells from the anaerobic p. major muscle are more sensitive than satellite cells from the aerobic b. femoris muscle to both hot and cold thermal stress during myogenic proliferation and differentiation. © 2016 Published by the American Physiological Society and The Physiological Society.
Scientific Publication
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