Advanced Search
Insect Molecular Biology
Singh, N.K., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Eliash, N., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Institute of Agroecology and Plant Health, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Stein, I., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Kamer, Y., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Ilia, Z., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Rafaeli, A., Department of Food Quality and Safety, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Soroker, V., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
The ectoparasitic mite Varroa destructor is one of the major threats to apiculture. Using a behavioural choice bioassay, we determined that phoretic mites were more successful in reaching a bee than reproductive mites, suggesting an energy trade-off between reproduction and host selection. We used both chemo-ecological and molecular strategies to identify the regulation of the olfactory machinery of Varroa and its association with reproduction. We focused on transcription regulation. Using primers designed to the conserved DNA binding region of transcription factors, we identified a gene transcript in V. destructor homologous to the pheromone receptor transcription factor (PRTF) gene of Pediculus humanus corporis. Quantitative PCR (qPCR) revealed that this PRTF-like gene transcript is expressed in the forelegs at higher levels than in the body devoid of forelegs. Subsequent comparative qPCR analysis showed that transcript expression was significantly higher in the phoretic as compared to the reproductive stage. Electrophysiological and behavioural studies revealed a reduction in the sensitivity of PRTF RNA interference-silenced mites to bee headspace, consistent with a reduction in the mites’ ability to reach a host. In addition, vitellogenin expression was stimulated in PRTF-silenced mites to similar levels as found in reproductive mites. These data shed light upon the regulatory mechanism of host chemosensing in V. destructor. © 2016 The Royal Entomological Society
Powered by ClearMash Solutions Ltd -
Volcani treasures
About
Terms of use
Identification and gene-silencing of a putative odorant receptor transcription factor in Varroa destructor: possible role in olfaction
25
Singh, N.K., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Eliash, N., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Institute of Agroecology and Plant Health, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Stein, I., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Kamer, Y., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Ilia, Z., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Rafaeli, A., Department of Food Quality and Safety, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Soroker, V., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Identification and gene-silencing of a putative odorant receptor transcription factor in Varroa destructor: possible role in olfaction
The ectoparasitic mite Varroa destructor is one of the major threats to apiculture. Using a behavioural choice bioassay, we determined that phoretic mites were more successful in reaching a bee than reproductive mites, suggesting an energy trade-off between reproduction and host selection. We used both chemo-ecological and molecular strategies to identify the regulation of the olfactory machinery of Varroa and its association with reproduction. We focused on transcription regulation. Using primers designed to the conserved DNA binding region of transcription factors, we identified a gene transcript in V. destructor homologous to the pheromone receptor transcription factor (PRTF) gene of Pediculus humanus corporis. Quantitative PCR (qPCR) revealed that this PRTF-like gene transcript is expressed in the forelegs at higher levels than in the body devoid of forelegs. Subsequent comparative qPCR analysis showed that transcript expression was significantly higher in the phoretic as compared to the reproductive stage. Electrophysiological and behavioural studies revealed a reduction in the sensitivity of PRTF RNA interference-silenced mites to bee headspace, consistent with a reduction in the mites’ ability to reach a host. In addition, vitellogenin expression was stimulated in PRTF-silenced mites to similar levels as found in reproductive mites. These data shed light upon the regulatory mechanism of host chemosensing in V. destructor. © 2016 The Royal Entomological Society
Scientific Publication
You may also be interested in