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Use of Nitrogen-15 Determinations of Purine Nitrogen Fraction of Digesta to Define Nitrogen Metabolism Traits in the Rumen
Year:
1991
Source of publication :
Journal of Dairy Science
Authors :
Aharoni, Yoav
;
.
Volume :
74
Co-Authors:


Tagari, H., Department of Animal Science, Faculty of Agriculture, Rehovot, 76-100, Israel

Facilitators :
From page:
2540
To page:
2547
(
Total pages:
8
)
Abstract:
A method for direct purine N isolation and determination was modified from the method of Zinn and Owens for rapid determination of purines. By this method, N derived from purines in digesta was measured and collected for 15N determinations. Measurements of purine N in 33 samples of ruminal contents and duodenal digesta of sheep and goats were compared with purine determinations in the same samples. The results showed that essentially all the N in the purines that could be isolated by the Zinn and Owens procedure also could be detected by N isolation and determination. Determinations of 15N in the NH3 N, NAN, and purine N fractions in a continuous culture in vitro showed significant differences in the 15N enrichment curves between diets based on either roasted or raw soybean meal. © 1991, American Dairy Science Association. All rights reserved.
Note:
Related Files :
Animal
cattle
metabolism
Nucleic acids
nucleic bases
purine nitrogen
Rumen
spectrophotometric procedure
Show More
Related Content
More details
DOI :
10.3168/jds.S0022-0302(91)78431-2
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24849
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:10
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Scientific Publication
Use of Nitrogen-15 Determinations of Purine Nitrogen Fraction of Digesta to Define Nitrogen Metabolism Traits in the Rumen
74


Tagari, H., Department of Animal Science, Faculty of Agriculture, Rehovot, 76-100, Israel

Use of Nitrogen-15 Determinations of Purine Nitrogen Fraction of Digesta to Define Nitrogen Metabolism Traits in the Rumen
A method for direct purine N isolation and determination was modified from the method of Zinn and Owens for rapid determination of purines. By this method, N derived from purines in digesta was measured and collected for 15N determinations. Measurements of purine N in 33 samples of ruminal contents and duodenal digesta of sheep and goats were compared with purine determinations in the same samples. The results showed that essentially all the N in the purines that could be isolated by the Zinn and Owens procedure also could be detected by N isolation and determination. Determinations of 15N in the NH3 N, NAN, and purine N fractions in a continuous culture in vitro showed significant differences in the 15N enrichment curves between diets based on either roasted or raw soybean meal. © 1991, American Dairy Science Association. All rights reserved.
Scientific Publication
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