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Induction of Rhizopus oryzae germination under starvation using host metabolites increases spore susceptibility to heat stress
Year:
2014
Source of publication :
Phytopathology
Authors :
Eshel, Dani
;
.
Kakongi, Nathan
;
.
Lichter, Amnon
;
.
Schneider, Avishai
;
.
Teper-Bamnolker, Paula
;
.
Turgeman, Tidhar
;
.
Vinokur, Yakov
;
.
Volume :
104
Co-Authors:
Turgeman, T., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Kakongi, N., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel, Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Schneider, A., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel, Department of Chemistry, Tel Aviv University, Tel Aviv, Israel
Vinokur, Y., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Teper-Bamnolker, P., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Carmeli, S., Department of Chemistry, Tel Aviv University, Tel Aviv, Israel
Levy, M., Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Skory, C.D., Renewable Product Technology Research Unit, NTL Center for Agricultural Utilization Research, Peoria, IL, United States
Lichter, A., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Eshel, D., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Facilitators :
From page:
240
To page:
247
(
Total pages:
8
)
Abstract:
Sweetpotato is a nutritional source worldwide. Soft rot caused by Rhizopus spp. is a major limiting factor in the storage of produce, rendering it potentially unsafe for human consumption. In this study, Rhizopus oryzae was used to develop a concept of postharvest disease control by weakening the pathogen through induction of spore germination under starvation conditions. We isolated the sweetpotato active fractions (SPAFs) that induce spore germination and used them at a low dose to enhance spore weakening caused by starvation. Germination in SPAF at 1 mg/ml weakened the pathogen spores by delaying their ability to form colonies on rich media and by increasing their sensitivity to heat stress. The weakening effect was also supported by reduced metabolic activity, as detected by Alarmar Blue fluorescent dye assays. Spores incubated with SPAF at 1 mg/ml showed DNA fragmentation in some of their nuclei, as observed by TUNEL assay. In addition, these spores exhibited changes in ultrastructural morphology (i.e., shrinkage of germ tubes, nucleus deformation, and vacuole formation) which are hallmarks of programmed cell death. We suggest that induction of spore germination under starvation conditions increases their susceptibility to stress and, therefore, might be considered a new strategy for pathogen control. © 2014 The American Phytopathological Society.
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More details
DOI :
10.1094/PHYTO-08-13-0245-R
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24884
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:10
You may also be interested in
Scientific Publication
Induction of Rhizopus oryzae germination under starvation using host metabolites increases spore susceptibility to heat stress
104
Turgeman, T., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Kakongi, N., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel, Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Schneider, A., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel, Department of Chemistry, Tel Aviv University, Tel Aviv, Israel
Vinokur, Y., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Teper-Bamnolker, P., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Carmeli, S., Department of Chemistry, Tel Aviv University, Tel Aviv, Israel
Levy, M., Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Skory, C.D., Renewable Product Technology Research Unit, NTL Center for Agricultural Utilization Research, Peoria, IL, United States
Lichter, A., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Eshel, D., Department of Postharvest Sciences of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, Bet-Dagan, Israel
Induction of Rhizopus oryzae germination under starvation using host metabolites increases spore susceptibility to heat stress
Sweetpotato is a nutritional source worldwide. Soft rot caused by Rhizopus spp. is a major limiting factor in the storage of produce, rendering it potentially unsafe for human consumption. In this study, Rhizopus oryzae was used to develop a concept of postharvest disease control by weakening the pathogen through induction of spore germination under starvation conditions. We isolated the sweetpotato active fractions (SPAFs) that induce spore germination and used them at a low dose to enhance spore weakening caused by starvation. Germination in SPAF at 1 mg/ml weakened the pathogen spores by delaying their ability to form colonies on rich media and by increasing their sensitivity to heat stress. The weakening effect was also supported by reduced metabolic activity, as detected by Alarmar Blue fluorescent dye assays. Spores incubated with SPAF at 1 mg/ml showed DNA fragmentation in some of their nuclei, as observed by TUNEL assay. In addition, these spores exhibited changes in ultrastructural morphology (i.e., shrinkage of germ tubes, nucleus deformation, and vacuole formation) which are hallmarks of programmed cell death. We suggest that induction of spore germination under starvation conditions increases their susceptibility to stress and, therefore, might be considered a new strategy for pathogen control. © 2014 The American Phytopathological Society.
Scientific Publication
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