Co-Authors:
Reuveni, O., ARO, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Golubowicz, S., ARO, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Abstract:
Studies with mango propagules of different cultivars were done in two main lines: I) small internodes for multishoot propagation via organogenesis and II) excised nucelli established in culture in order to obtain embryogenic cultures. Media composition were basically those reported in the literature and our own modifications. Cultures were incubated in most cases in dark at 28°C. When small intemodes were cultured, browning of the cultures and endophytic contamination were the two main factors which limited their establishment and proliferation. Different media and supplements including activated charcoal and specific antibiotics did not improved the efficiency of shoot formation in established cultures. Ovules were isolated from seven different cultivars at five different stages of flowers and fruitlets development from the end of May to the end of June 1990. Survival rate of non-contaminated explants and the rate of embryogenic cultures obtained was determined by the age of the fruitlet. An advantage was found in two media with the following composition (mg/l):(l)- macroelements of MS (1/2 strength), thiamine HCI(0.5), glutamine(400), ascorbic acid(100), Sucrose (6%), (2)-macroelements of WPM, thiamine IICI( 1 .0), sucrose (3%). Common supplements were added to both media of MS microelements: 2.4.D(50), activated charcoal (0.25%) and Agar(0.65%). Highly proliferate embryogenic cultures were obtained in some cultivars. In few other cases non-embryogenic cultures, at the initiation stage, started to form embryos at a later stage. Once these embryogenic cultures were formed they continued to proliferate as embryogenic cultures for several years. A further study is required to bring the embryos to a high efficiency of maturation and germination which will enable to use the technique for mass propagation of mango. © ISHS.
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