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Positive and negative feedback loops affect the transcription of IME1, a positive regulator of meiosis in Saccharomyces cerevisiae
Year:
1995
Source of publication :
Developmental Genetics
Authors :
Sherman, Amir
;
.
Volume :
16
Co-Authors:
Shefer‐Vaida, M., Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel
Sherman, A., Whitehead Institute, Cambridge, Massachusetts, United States
Ashkenazi, T., Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel
Robzyk, K., Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel
Kassir, Y., Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel
Facilitators :
From page:
219
To page:
228
(
Total pages:
10
)
Abstract:
The IME1 gene of Saccharomyces cerevisiae encodes a transcription factor that is required for the expression of meiosis‐specific genes. Like many of the genes it regulates, IME1 itself is expressed according to the following complex pattern: barely detectable levels during vegetative growth, and high induced levels under starvation conditions, followed by a subsequent decline in the course of meiosis. This report examines the influence of Ime1 protein on its own expression, demonstrating feedback regulation. Disruption of either IME1 or IME2 leads to constantly increasing levels of Ime1‐lacZ expression, under meiotic conditions. This apparent negative regulation is due to cis elements in the IME1 upstream region, which confer transient meiotic expression to heterologous promoter‐less genes. A specific DNA/protein complex, whose level is transiently increased under meiotic conditions, is detected on this element. In ime1− diploids, the level of this DNA/protein complex increases, without any decline. These results indicate that the transient expression of IME1 is apparently due to transcriptional regulation. This report also presents evidence suggesting that Ime 1p is directly responsible for regulating its own transcription. Positive feedback regulation in mitotic conditions is suggested by the observation that overexpression of Ime 1p leads to increased levels of IME1‐lacZ. Negative autoregulation in meiotic cultures is demonstrated by the observation that a specific point mutation in IME1, ime 1–3, permits expression of meiosis‐specific genes, as well as induction of meiosis, but is defective in negative‐feedback regulation of IME1. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc.
Note:
Related Files :
DNA flanking region
DNA protein complex
gene expression regulation
Genes, Fungal
Nuclear Proteins
Show More
Related Content
More details
DOI :
10.1002/dvg.1020160302
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24921
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
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Scientific Publication
Positive and negative feedback loops affect the transcription of IME1, a positive regulator of meiosis in Saccharomyces cerevisiae
16
Shefer‐Vaida, M., Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel
Sherman, A., Whitehead Institute, Cambridge, Massachusetts, United States
Ashkenazi, T., Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel
Robzyk, K., Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel
Kassir, Y., Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel
Positive and negative feedback loops affect the transcription of IME1, a positive regulator of meiosis in Saccharomyces cerevisiae
The IME1 gene of Saccharomyces cerevisiae encodes a transcription factor that is required for the expression of meiosis‐specific genes. Like many of the genes it regulates, IME1 itself is expressed according to the following complex pattern: barely detectable levels during vegetative growth, and high induced levels under starvation conditions, followed by a subsequent decline in the course of meiosis. This report examines the influence of Ime1 protein on its own expression, demonstrating feedback regulation. Disruption of either IME1 or IME2 leads to constantly increasing levels of Ime1‐lacZ expression, under meiotic conditions. This apparent negative regulation is due to cis elements in the IME1 upstream region, which confer transient meiotic expression to heterologous promoter‐less genes. A specific DNA/protein complex, whose level is transiently increased under meiotic conditions, is detected on this element. In ime1− diploids, the level of this DNA/protein complex increases, without any decline. These results indicate that the transient expression of IME1 is apparently due to transcriptional regulation. This report also presents evidence suggesting that Ime 1p is directly responsible for regulating its own transcription. Positive feedback regulation in mitotic conditions is suggested by the observation that overexpression of Ime 1p leads to increased levels of IME1‐lacZ. Negative autoregulation in meiotic cultures is demonstrated by the observation that a specific point mutation in IME1, ime 1–3, permits expression of meiosis‐specific genes, as well as induction of meiosis, but is defective in negative‐feedback regulation of IME1. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc.
Scientific Publication
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