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Light affects motility and infectivity of Agrobacterium tumefaciens
Year:
2008
Source of publication :
Environmental Microbiology
Authors :
Rosen, Ran
;
.
Volume :
10
Co-Authors:
Oberpichler, I., Freie UniversitäBerlin, Pflanzenphysiologie, Königin-Luise-Straße 12-16, 14195 Berlin, Germany, Universität Karlsruhe, Botanik 1, Kaiserstr. 2, 76131 Karlsruhe, Germany
Rosen, R., Maiman Institute for Proteome Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel, Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel, Agentek (1987) Ltd., Atidim Scientific Park, Tel Aviv, Israel
Rasouly, A., Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Vugman, M., Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Ron, E.Z., Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Lamparter, T., Freie UniversitäBerlin, Pflanzenphysiologie, Königin-Luise-Straße 12-16, 14195 Berlin, Germany, Universität Karlsruhe, Botanik 1, Kaiserstr. 2, 76131 Karlsruhe, Germany
Facilitators :
From page:
2020
To page:
2029
(
Total pages:
10
)
Abstract:
Response to changes in light conditions involves a variety of receptors that can modulate gene expression, enzyme activity and/or motility. For the study of light-regulated effects of Agrobacterium tumefaciens, we used a global analysis approach - proteomics - and compared the protein patterns of dark- and light-grown bacteria. These analyses revealed a significant reduction of FlaA and FlaB - proteins of the flagellum - when the cells were grown in light. The light effect was confirmed by SDS-PAGE with isolated flagella. Quantitative PCR experiments showed a 10-fold increase of the transcription level of flaA, flaB and flaC within 20 min after the transfer from light to darkness. Electron microscopy revealed that these molecular events result in a light-induced reduction of the number of flagella per cell. These changes have major physilogical consequences regarding motility, which is considerably reduced with exposure to light. The inhibitory effect of light on the motility is not unique to A. tumefaciens and was also seen in other species of the Rhizobiaceae. Previous studies suggested that the flagella function is significant for bacteria-plant interactions and bacterial virulence. In our studies, light reduced the attachment of A. tumefaciens to tomato roots and the virulence of the bacteria in a cucumber infection assay. © 2008 Society for Applied Microbiology and Blackwell Publishing Ltd.
Note:
Related Files :
Cucumis sativus
light
metabolism
Microbiology
Plant Disease
Plant Diseases
proteomics
virulence
Show More
Related Content
More details
DOI :
10.1111/j.1462-2920.2008.01618.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24927
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
Scientific Publication
Light affects motility and infectivity of Agrobacterium tumefaciens
10
Oberpichler, I., Freie UniversitäBerlin, Pflanzenphysiologie, Königin-Luise-Straße 12-16, 14195 Berlin, Germany, Universität Karlsruhe, Botanik 1, Kaiserstr. 2, 76131 Karlsruhe, Germany
Rosen, R., Maiman Institute for Proteome Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel, Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel, Agentek (1987) Ltd., Atidim Scientific Park, Tel Aviv, Israel
Rasouly, A., Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Vugman, M., Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Ron, E.Z., Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Lamparter, T., Freie UniversitäBerlin, Pflanzenphysiologie, Königin-Luise-Straße 12-16, 14195 Berlin, Germany, Universität Karlsruhe, Botanik 1, Kaiserstr. 2, 76131 Karlsruhe, Germany
Light affects motility and infectivity of Agrobacterium tumefaciens
Response to changes in light conditions involves a variety of receptors that can modulate gene expression, enzyme activity and/or motility. For the study of light-regulated effects of Agrobacterium tumefaciens, we used a global analysis approach - proteomics - and compared the protein patterns of dark- and light-grown bacteria. These analyses revealed a significant reduction of FlaA and FlaB - proteins of the flagellum - when the cells were grown in light. The light effect was confirmed by SDS-PAGE with isolated flagella. Quantitative PCR experiments showed a 10-fold increase of the transcription level of flaA, flaB and flaC within 20 min after the transfer from light to darkness. Electron microscopy revealed that these molecular events result in a light-induced reduction of the number of flagella per cell. These changes have major physilogical consequences regarding motility, which is considerably reduced with exposure to light. The inhibitory effect of light on the motility is not unique to A. tumefaciens and was also seen in other species of the Rhizobiaceae. Previous studies suggested that the flagella function is significant for bacteria-plant interactions and bacterial virulence. In our studies, light reduced the attachment of A. tumefaciens to tomato roots and the virulence of the bacteria in a cucumber infection assay. © 2008 Society for Applied Microbiology and Blackwell Publishing Ltd.
Scientific Publication
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