אסיף מאגר המחקר החקלאי

Transgenic Expression of the Dicotyledonous Pattern Recognition Receptor EFR in Rice Leads to Ligand-Dependent Activation of Defense Responses

Year:

2015

Source of publication :

Authors :

Volume :

11

Co-Authors:

Schwessinger, B., Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, United States, Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States
Bahar, O., Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, United States, Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Thomas, N., Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, United States
Holton, N., The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom
Nekrasov, V., The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom
Ruan, D., Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, United States, Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States
Canlas, P.E., Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, United States, Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States
Daudi, A., Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, United States, bio-protocol, Palo Alto, CA, United States
Petzold, C.J., Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States
Singan, V.R., Department of Energy Joint Genome Institute, Walnut Creek, CA, United States
Kuo, R., Department of Energy Joint Genome Institute, Walnut Creek, CA, United States
Chovatia, M., Department of Energy Joint Genome Institute, Walnut Creek, CA, United States
Daum, C., Department of Energy Joint Genome Institute, Walnut Creek, CA, United States
Heazlewood, J.L., Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States
Zipfel, C., The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom
Ronald, P.C., Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, United States, Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States

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Abstract:

Plant plasma membrane localized pattern recognition receptors (PRRs) detect extracellular pathogen-associated molecules. PRRs such as Arabidopsis EFR and rice XA21 are taxonomically restricted and are absent from most plant genomes. Here we show that rice plants expressing EFR or the chimeric receptor EFR::XA21, containing the EFR ectodomain and the XA21 intracellular domain, sense both Escherichia coli- and Xanthomonas oryzae pv. oryzae (Xoo)-derived elf18 peptides at sub-nanomolar concentrations. Treatment of EFR and EFR::XA21 rice leaf tissue with elf18 leads to MAP kinase activation, reactive oxygen production and defense gene expression. Although expression of EFR does not lead to robust enhanced resistance to fully virulent Xoo isolates, it does lead to quantitatively enhanced resistance to weakly virulent Xoo isolates. EFR interacts with OsSERK2 and the XA21 binding protein 24 (XB24), two key components of the rice XA21-mediated immune response. Rice-EFR plants silenced for OsSERK2, or overexpressing rice XB24 are compromised in elf18-induced reactive oxygen production and defense gene expression indicating that these proteins are also important for EFR-mediated signaling in transgenic rice. Taken together, our results demonstrate the potential feasibility of enhancing disease resistance in rice and possibly other monocotyledonous crop species by expression of dicotyledonous PRRs. Our results also suggest that Arabidopsis EFR utilizes at least a subset of the known endogenous rice XA21 signaling components.

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