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Plant physiology (source)
Bental, M., Department of Isotope Research, The Weizmann Institute of Science, Rehovot, Israel
Oren-Shamir, M., Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel
Avron, M., Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel
Degani, H., Department of Isotope Research, The Weizmann Institute of Science, Rehovot, Israel
The intracellular phosphorus and carbon metabolites in the halotolerant alga Dunaliella salina adapted to different salinities were monitored in living cells by31P-and13C-nuclear magnetic resonance (NMR) spectroscopy. The13C-NMR studies showed that the composition of the visible intracellular carbon metabolites other than glycerol is not signiflcantly affected by the salinity of the growth medium. The T1 relaxation rates of the13C-glycerol signals in intact cells were enhanced with increasing salinity of the growth medium, in parallel to the expected increase in the intracellular viscosity due to the increase in intracellular glycerol. The31P-NMR studies showed that cells adapted to the various salinities contained inorganic phosphate, phosphomonoesters, high energy phosphatecompounds, and long chain polyphosphates. In addition, cells grown in media containing up to 1 molar NaCI contained tripolyphosphates. The tripolyphosphate content was also controlled by the availability of inorganic phosphate during cell growth. Phosphate-depleted D. salina contained no detectable tripolyphosphate signal. Excess phosphate, however, did not result in the appearance of tripolyphosphate in31P-NMR spectra of cells adapted to high (>1.5 molar NaCI) salinities. © 1988 American Society of Plant Biologists. All rights reserved.
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31P and13C-NMR studies of the phosphorus and carbon metabolites in the halotolerant alga, Dunaliella salina
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Bental, M., Department of Isotope Research, The Weizmann Institute of Science, Rehovot, Israel
Oren-Shamir, M., Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel
Avron, M., Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel
Degani, H., Department of Isotope Research, The Weizmann Institute of Science, Rehovot, Israel
31P and13C-NMR studies of the phosphorus and carbon metabolites in the halotolerant alga, Dunaliella salina
The intracellular phosphorus and carbon metabolites in the halotolerant alga Dunaliella salina adapted to different salinities were monitored in living cells by31P-and13C-nuclear magnetic resonance (NMR) spectroscopy. The13C-NMR studies showed that the composition of the visible intracellular carbon metabolites other than glycerol is not signiflcantly affected by the salinity of the growth medium. The T1 relaxation rates of the13C-glycerol signals in intact cells were enhanced with increasing salinity of the growth medium, in parallel to the expected increase in the intracellular viscosity due to the increase in intracellular glycerol. The31P-NMR studies showed that cells adapted to the various salinities contained inorganic phosphate, phosphomonoesters, high energy phosphatecompounds, and long chain polyphosphates. In addition, cells grown in media containing up to 1 molar NaCI contained tripolyphosphates. The tripolyphosphate content was also controlled by the availability of inorganic phosphate during cell growth. Phosphate-depleted D. salina contained no detectable tripolyphosphate signal. Excess phosphate, however, did not result in the appearance of tripolyphosphate in31P-NMR spectra of cells adapted to high (>1.5 molar NaCI) salinities. © 1988 American Society of Plant Biologists. All rights reserved.
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