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Preparation of viable isolated bovine parathyroid cells
Year:
1976
Source of publication :
Endocrinology
Authors :
Hurvitz, Shmuel (Animal science)
;
.
Volume :
99
Co-Authors:
Brown, E.M., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, Bethesda, MD, 20014, United States, Digestive Diseases, National Institutes of Health, Bethesda, MD, 20014, United States
Hurwitz, S., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, Bethesda, MD, 20014, United States, Digestive Diseases, National Institutes of Health, Bethesda, MD, 20014, United States
Aurbach, G.D., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, Bethesda, MD, 20014, United States, Digestive Diseases, National Institutes of Health, Bethesda, MD, 20014, United States
Facilitators :
From page:
1582
To page:
1588
(
Total pages:
7
)
Abstract:
A method is described for the preparation of isolated bovine parathyroid cells. Fresh, minced bovine parathyroid tissue is incubated with 2 mg/ml collagenase and 50 μg/ml DNAse for 1 h at 37 C with periodic pipetting. Parathyroid cells are separated from contaminating fat cells and red cells by low speed centrifugation. The resulting cell preparation is indistinguishable from parathyroid cells in intact bovine glands by light and electron microscopy. Hormone release is linear for at least 3 h at both high (2.0 ITIM) and low (0.5 mM) calcium concentrations and is inversely proportional to divalent cation concentration between 0.3 mM and 1.0 mM calcium as has been observed previously both in vivo and in vitro. As in previous studies, hormone release is also stimulated up to 2-fold by 106M (-)isoproterenol, an effect blocked by 10-6M (-)propranolol, suggesting a β-adrenergic effect. Such a cell preparation should be useful for studying hormone binding and effects on cyclic nucleotides and cellular transport phenomena in both normal and abnormal tissue. © 1976 by The Endocrine Society.
Note:
Related Files :
Animal
cattle
electron microscopy
Histology
Parathyroid Glands
Parathyroid Hormones
propranolol
radioisotope
Show More
Related Content
More details
DOI :
10.1210/endo-99-6-1582
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
25318
Last updated date:
21/08/2022 07:45
Creation date:
17/04/2018 00:14
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Scientific Publication
Preparation of viable isolated bovine parathyroid cells
99
Brown, E.M., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, Bethesda, MD, 20014, United States, Digestive Diseases, National Institutes of Health, Bethesda, MD, 20014, United States
Hurwitz, S., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, Bethesda, MD, 20014, United States, Digestive Diseases, National Institutes of Health, Bethesda, MD, 20014, United States
Aurbach, G.D., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, Bethesda, MD, 20014, United States, Digestive Diseases, National Institutes of Health, Bethesda, MD, 20014, United States
Preparation of viable isolated bovine parathyroid cells
A method is described for the preparation of isolated bovine parathyroid cells. Fresh, minced bovine parathyroid tissue is incubated with 2 mg/ml collagenase and 50 μg/ml DNAse for 1 h at 37 C with periodic pipetting. Parathyroid cells are separated from contaminating fat cells and red cells by low speed centrifugation. The resulting cell preparation is indistinguishable from parathyroid cells in intact bovine glands by light and electron microscopy. Hormone release is linear for at least 3 h at both high (2.0 ITIM) and low (0.5 mM) calcium concentrations and is inversely proportional to divalent cation concentration between 0.3 mM and 1.0 mM calcium as has been observed previously both in vivo and in vitro. As in previous studies, hormone release is also stimulated up to 2-fold by 106M (-)isoproterenol, an effect blocked by 10-6M (-)propranolol, suggesting a β-adrenergic effect. Such a cell preparation should be useful for studying hormone binding and effects on cyclic nucleotides and cellular transport phenomena in both normal and abnormal tissue. © 1976 by The Endocrine Society.
Scientific Publication
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