Co-Authors:
Russo, S., Inst. of Microbiology and Immunology, Fac. of Vet. Med., Via Celoria, 10, 20133 Milan, Italy
Montermini, L., Department of Neurology, Ctr. de Rech. L.C. Simard, Hop. N., Montreal, Que. H2L 4MI, Canada
Berkovitz-Siman-Tov, R., Dept. of Post-Harvest Sci. of F., ARO, the Volcani Center, Bet Dagan 50250, Israel
Ponti, W., Inst. of Microbiology and Immunology, Fac. of Vet. Med., Via Celoria, 10, 20133 Milan, Italy
Poli, G., Inst. of Microbiology and Immunology, Fac. of Vet. Med., Via Celoria, 10, 20133 Milan, Italy
Abstract:
The gp51-p30 glycoprotein constituting BLV envelope was expressed in Sf-21 insect cells by means of recombinant baculoviruses. Post-infection cell lysates were analyzed, in order to define the immunologic reactivity of recombinant products. Oligosaccharide chains, containing N-acetylglucosamine, mannose, galactose and sialic acid were found on recombinant gp51-p30. In order to investigate the timing of transcription and translation of the glycoprotein, kinetic assays were carried out on cell lysates and directly in situ on Sf-21 cells during the course of baculovirus infection. The use of different solubilizing reagents was also evaluated in order to rescue recombinant glycoprotein from its subcellular location. Copyright (C) 1998 Federation of European Biochemical Societies.
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