Co-Authors:
Lewinsohn, E., Arom., Med. and Spice Crops Unit, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Dudai, N., Arom., Med. and Spice Crops Unit, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Tadmor, Y., Corn Breeding Unit, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Katzir, I., Arom., Med. and Spice Crops Unit, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Ravid, U., Arom., Med. and Spice Crops Unit, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Putievsky, E.
Joel, D.M., Department of Weed Research, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Abstract:
Lemongrasses (Cymbopogon spp., Poaceae) are a group of commercially important C4 tropical grasses. Their leaves contain up to 1.5% (d.wt) essential oils with a typical lemon-like aroma, consisting mainly of citral (a mixture of the isomeric acyclic monoterpene aldehydes geranial and neral). To specifically locate the sites of citral accumulation in lemongrass we employed Schiff's reagent, which reacts with aldehydes and gives a purple-red coloration with citral. Using this technique, single oil-accumulating cells were detected in the adaxial side of leaf mesophyll, commonly adjacent to non-photosynthetic tissue, and between vascular bundles. Cell walls of these oil cells are lignified. Our results suggest that citral accumulation takes place in individual oil cells within the leaf tissues.