אסיף מאגר המחקר החקלאי

Regeneration in vitro from the hypocotyl of Cucumis species produces almost exclusively diploid shoots, and does not require light

Year:

2003

Source of publication :

Authors :

Volume :

38

Co-Authors:

Curuk, S., Department of Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel, Laboratory of Plant Biotechnology, University of Cukorova, Adana, Turkey, Univ. of Mustafa Kemal, Faculty of Agriculture, Dept. of Horticulture, 31034 Antakya-HATAY, Turkey
Ananthakrishnan, G., Department of Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel, Citrus Research and Education Center, Univ. of Florida, 700 Experiment Station Rd., Lake Alfred, FL 33850, United States
Singer, S., Department of Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Xia, X., Department of Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Elman, C., Department of Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Nestel, D., Department of Entomology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Cetiner, S., Laboratory of Plant Biotechnology, University of Cukorova, Adana, Turkey, Laboratory of Plant Biotechnology, University of Cukorova, Adana, Turkey
Gaba, V., Department of Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel

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Abstract:

Hypocotyl explants of three cultivars of melon (Cucumis melo L.) (cvs. Revigal, Topmark and Kirkagac), and a cucumber (C. sativus L. cv. Taoz) rapidly directly regenerated multiple shoots on Murashige and Skoog medium augmented with 4.4 μM benzyladenine. Regeneration from the hypocotyl resulted in nearly 100% diploid shoots, whereas regeneration from the cotyledons resulted in 40% to 70% polyploid regenerants. Regeneration from cotyledon explants of melon cv. Revigal required light, whereas regeneration from hypocotyl explants of melon cv. Revigal occurred in both light and darkness. Direct regeneration also occurred from the hypocotyl of cucumber cv. Taoz in both light and darkness, even though cotyledonary explants did not regenerate buds or shoots under the same conditions. This is the first report of regeneration from the Cucumis genus producing a fully diploid plant population.

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