Co-Authors:
Fridman, E., Department of Field and Vegetable Crops, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Liu, Y.S., Department of Field and Vegetable Crops, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel, Boyce Thompson Institute for Plant Research, Cornell University, Itacha, NY 14853, United States
Carmel-Goren, L., Department of Field and Vegetable Crops, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Gur, A., Department of Field and Vegetable Crops, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Shoresh, M., Department of Field and Vegetable Crops, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Pleban, T., Department of Field and Vegetable Crops, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Eshed, Y., Department of Field and Vegetable Crops, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel, Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Zamir, D., Department of Field and Vegetable Crops, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Abstract:
Congenic lines that differ in a single defined chromosome segment are useful for the study of complex phenotypes, as they allow isolation of the effect of a particular quantitative trait locus (QTL) from those of the entire genome. We conducted high-resolution QTL mapping of a 9-cM introgression, originating from the wild tomato species Lycopersicon pennellii, in two extremely different genetic and physiological backgrounds. In the "indeterminate" glasshouse background we identified only a single QTL (Brix9-2-5) that affects the total soluble solids of the fruit [mainly sugars, measured in Brix units (B)]. This QTL was previously delimited within the gene for an apoplastic invertase, Lin5, that modulates sugar partitioning to the fruit. Analysis of the effects of the same chromosome segment in "determinate", open-field tomatoes, revealed two QTLs, 0.3 cM apart: the fruit-specific Brix9-2-5 that affects B only, and the shoot-specific PW9-2-5, which accounts for an altered growth habit resulting in increases in plant weight, yield, and B. This study highlights the power of the congenic approach for dissecting developmental pathways leading to complex phenotypes.
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