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Journal of Bacteriology
Sela, S., Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
Yogev, D., Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
Razin, S., Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
Bercovier, H., Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
The conservation and duplication of the tuf gene encoding the elongation factor EF-Tu were used to define phylogenetic relationships among eubacteria. When the tufA gene of Escherichia coli was used as a probe in hybridization experiments, duplicate tuf genes were found in gram-negative bacteria from three major phyla: purple bacteria, bacteroides, and cyanobacteria. Only a single copy of tuf was found in gram-positive bacteria, including mycobacteria and mycoplasmas. Gram-positive clostridia were found to carry two copies of tuf.
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Duplication of the tuf gene: a new insight into the phylogeny of eubacteria.
171
Sela, S., Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
Yogev, D., Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
Razin, S., Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
Bercovier, H., Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
Duplication of the tuf gene: a new insight into the phylogeny of eubacteria.
The conservation and duplication of the tuf gene encoding the elongation factor EF-Tu were used to define phylogenetic relationships among eubacteria. When the tufA gene of Escherichia coli was used as a probe in hybridization experiments, duplicate tuf genes were found in gram-negative bacteria from three major phyla: purple bacteria, bacteroides, and cyanobacteria. Only a single copy of tuf was found in gram-positive bacteria, including mycobacteria and mycoplasmas. Gram-positive clostridia were found to carry two copies of tuf.
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