Sucrose phosphate synthase, sucrose synthase, and invertase activities in developing fruit of Lycopersicon esculentum mill. and the sucrose accumulating Lycopersicon hirsutum Humb. and Bonpl.

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Sucrose phosphate synthase, sucrose synthase, and invertase activities in developing fruit of Lycopersicon esculentum mill. and the sucrose accumulating Lycopersicon hirsutum Humb. and Bonpl.

Year: 

1991

Source of publication :

Plant physiology (source)

Authors :

Miron, Dafna

;

Schaffer, Arthur

.

Volume :

Co-Authors: 

Miron, D., Department of Vegetable Crops, Agric. Res. Org.-The Volcani Center, Bet Dagan, 50250, Israel
Schaffer, A.A., Department of Vegetable Crops, Agric. Res. Org.-The Volcani Center, Bet Dagan, 50250, Israel

From page: 

623

To page: 

627

(

Total pages: 

5

)

Link :

Sucrose phosphate synthase, sucrose synthase, and invertase activities in developing fruit of Lycopersicon esculentum mill. and the sucrose accumulating Lycopersicon hirsutum Humb. and Bonpl.

Abstract: 

The green-fruited Lycopersicon hirsutum Humb. and Bonpl. accumulated sucrose to concentrations of about 118 micromoles per gram fresh weight during the final stages of development. In comparison, Lycopersicon esculentum Mill. cultivars contained less than 15 micromoles per gram fresh weight of sucrose at the ripe stage. Glucose and fructose levels remained relatively constant throughout development in L. hirsutum at 22 to 50 micromoles per gram fresh weight each. Starch content was low even at early stages of development, and declined further with development. Soluble acid invertase (EC 3.2.1.26) activity declined concomitant with the rise in sucrose content. Acid invertase activity, which was solubilized in 1 molar NaCI (presumably cell-wall bound), remained constant throughout development (about 3 micromoles of reducing sugars (per gram fresh weight) per hour. Sucrose phosphate synthase (EC 2.4.1.14) activity was present at about 5 micromoles of sucrose (per gram fresh weight) per hour even at early stages of development, and increased sharply to about 40 micromoles of sucrose (per gram fresh weight) per hour at the final stages of development studied, parallel to the rise in sucrose content. In comparison, sucrose phosphate synthase activity in L. esculentum remained low throughout development. The possible roles of the sucrose metabolizing enzymes in determining sucrose accumulation are discussed.