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Characterization of viral siRNA populations in honey bee colony collapse disorder
Year:
2014
Source of publication :
Virology
Authors :
Chejanovsky, Nor
;
.
Ophir, Ron
;
.
Schwager, Michal Sharabi
;
.
Volume :
454-455
Co-Authors:
Chejanovsky, N., Entomology Department, Institute of Plant Protection, ARO, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Ophir, R., Genomics Department, Institute of Plant Sciences, ARO, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Schwager, M.S., Genomics Department, Institute of Plant Sciences, ARO, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Slabezki, Y., Beekeeping Division, Extension Service, Israeli Ministry of Agriculture, POB 6, Bet Dagan 50250, Israel
Grossman, S., Entomology Department, Institute of Plant Protection, ARO, The Volcani Center, POB 6, Bet Dagan 50250, Israel, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew, University of Jerusalem, Rehovot 76100, Israel
Cox-Foster, D., Entomology Department, PennState University, University Park 16802, United States
Facilitators :
From page:
176
To page:
183
(
Total pages:
8
)
Abstract:
Colony Collapse Disorder (CCD), a special case of collapse of honey bee colonies, has resulted in significant losses for beekeepers. CCD-colonies show abundance of pathogens which suggests that they have a weakened immune system. Since honey bee viruses are major players in colony collapse and given the important role of viral RNA interference (RNAi) in combating viral infections we investigated if CCD-colonies elicit an RNAi response. Deep-sequencing analysis of samples from CCD-colonies from US and Israel revealed abundant small interfering RNAs (siRNA) of 21-22 nucleotides perfectly matching the Israeli acute paralysis virus (IAPV), Kashmir virus and Deformed wing virus genomes. Israeli colonies showed high titers of IAPV and a conserved RNAi-pattern of matching the viral genome. That was also observed in sample analysis from colonies experimentally infected with IAPV. Our results suggest that CCD-colonies set out a siRNA response that is specific against predominant viruses associated with colony losses. © 2014 Elsevier Inc.
Note:
Related Files :
animal experiment
animal model
Animals
bees
colony collapse disorder
immune system
Israel
virus infection
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More details
DOI :
10.1016/j.virol.2014.02.012
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
25682
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:16
Scientific Publication
Characterization of viral siRNA populations in honey bee colony collapse disorder
454-455
Chejanovsky, N., Entomology Department, Institute of Plant Protection, ARO, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Ophir, R., Genomics Department, Institute of Plant Sciences, ARO, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Schwager, M.S., Genomics Department, Institute of Plant Sciences, ARO, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Slabezki, Y., Beekeeping Division, Extension Service, Israeli Ministry of Agriculture, POB 6, Bet Dagan 50250, Israel
Grossman, S., Entomology Department, Institute of Plant Protection, ARO, The Volcani Center, POB 6, Bet Dagan 50250, Israel, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew, University of Jerusalem, Rehovot 76100, Israel
Cox-Foster, D., Entomology Department, PennState University, University Park 16802, United States
Characterization of viral siRNA populations in honey bee colony collapse disorder
Colony Collapse Disorder (CCD), a special case of collapse of honey bee colonies, has resulted in significant losses for beekeepers. CCD-colonies show abundance of pathogens which suggests that they have a weakened immune system. Since honey bee viruses are major players in colony collapse and given the important role of viral RNA interference (RNAi) in combating viral infections we investigated if CCD-colonies elicit an RNAi response. Deep-sequencing analysis of samples from CCD-colonies from US and Israel revealed abundant small interfering RNAs (siRNA) of 21-22 nucleotides perfectly matching the Israeli acute paralysis virus (IAPV), Kashmir virus and Deformed wing virus genomes. Israeli colonies showed high titers of IAPV and a conserved RNAi-pattern of matching the viral genome. That was also observed in sample analysis from colonies experimentally infected with IAPV. Our results suggest that CCD-colonies set out a siRNA response that is specific against predominant viruses associated with colony losses. © 2014 Elsevier Inc.
Scientific Publication
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