Co-Authors:
Clark, E., Department of Biology, Gilmer Hall, University of Virginia, Charlottesville, 22901, VA, United States
Schnabelrauch, L., Department of Horticulture, Michigan State University, East Lansing, 48824, MI, United States
Hanson, M.R., Department of Biology, Gilmer Hall, University of Virginia, Charlottesville, 22901, VA, United States
Sink, K.C., Department of Horticulture, Michigan State University, East Lansing, 48824, MI, United States
Abstract:
The chloroplast (cp) and mitochondrial (mt) DNAs of Petunia somatic hybrid plants, which were derived from the fusion of wild-type P. parodii protoplasts with albino P. inflata protoplasts, were analyzed by endonuclease restriction and Southern blot hybridization. Using 32P-labelled probes that distinguished the two parental cpDNAs at a BamH1 site and at a HpaII site, only the P. parodii chloroplast genome was detected in the 10 somatic hybrid plants analyzed. To examine whether cytoplasmic mixing had resulted in rearrangement of the mitochondrial genome in the somatic hybrids, restriction patterns of purified somatic hybrid and parental mtDNAs were analyzed. Approximately 87% of those restriction fragments which distinguish the two parental genomes are P. inflata-specific. Restriction patterns of the somatic hybrid mtDNAs differ both from the parental patterns and from each other, suggesting that an interaction occurred between the parental mitochondrial genomes in the somatic fusion products which resulted in generation of the novel mtDNA patterns. Southern blot hybridization substantiates this conclusion. In addition, somatic hybrid lines derived from the same fusion product were observed to differ in mtDNA restriction pattern, reflecting a differential sorting-out of mitochondrial genomes at the time the plants were regenerated. © 1986 Springer-Verlag.
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