אסיף מאגר המחקר החקלאי

Effect of heat shock treatment on stress tolerance and biocontrol efficacy of Metschnikowia fructicola

Year:

2011

Source of publication :

Authors :

Volume :

76

Co-Authors:

Liu, J., US Department of Agriculture-Agricultural Research Service (USDA-ARS), Kearneysville, WV, United States, Institute of Botany, Chinese Academy of Sciences, Beijing, China
Wisniewski, M., US Department of Agriculture-Agricultural Research Service (USDA-ARS), Kearneysville, WV, United States
Droby, S., Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, Israel
Tian, S., Institute of Botany, Chinese Academy of Sciences, Beijing, China
Hershkovitz, V., Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, Israel
Tworkoski, T., US Department of Agriculture-Agricultural Research Service (USDA-ARS), Kearneysville, WV, United States

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Abstract:

The effect of high temperature and oxidative stress on the cell viability of the yeast antagonist, Metschnikowia fructicola was determined. A mild heat shock (HS) pretreatment (30min at 40°C) improved the tolerance of M. fructicola to subsequent high temperature (45°C, 20-30min) and oxidative stress (0.4molL-1 hydrogen peroxide, 20-60min). HS-treated yeast cells showed less accumulation of reactive oxygen species (ROS) than nontreated cells in response to both stresses. Additionally, HS-treated yeast exhibited significantly greater (P<0.0001) biocontrol activity against Penicillium expansum and a significantly faster (P<0.0001) growth rate in wounds of apple fruits stored at 25°C compared with the performance of untreated yeast cells. Transcription of a trehalose-6-phosphate synthase gene (TPS1) was upregulated in response to HS and trehalose content also increased. Results indicate that the higher levels of trehalose induced by the HS may contribute to an improvement in ROS scavenging, stress tolerance, population growth in apple wounds and biocontrol activity of M. fructicola. FEMS Microbiology Ecology © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. No claim to original US government works.

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