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Analytical Chemistry
Altstein, M., Department of Cell Biology and Neurosciences, University of California Riverside, Boyce Hall 5419, Riverside, CA 92521-0146, United States, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Bronshtein, A., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Glattstein, B., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Zeichner, A., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Tamiri, T., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Almog, J., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
A highly sensitive immunochemical method for immunoaffinity purification (IAP) and detection of trace amounts of TNT was developed on the basis of antibodies (Abs) in a ceramic matrix (sol-gel). The study resulted in: (i) a highly sensitive and reproducible TNT ELISA (I50 and I20 values of 0.4 ± 0.09 ppb and 0.12 ± 0.03 ppb, respectively, n = 12), which is highly specific to TNT; and (ii) successful entrapment of the Abs that bound free analyte from solution. Binding was found to be highly reproducible, dose dependent, and only slightly (1.2-1.8-fold) lower than that in solution. The entrapped Abs did not leach from the matrix and were tolerant of absolute ethanol, acetone, and acetonitrile. Bound analytes could be easily eluted from the sol-gel matrix at high recoveries. The sol-gel-based IAP method described above introduces a simple one-step procedure that has a high potential to serve as a suitable and convenient immunochromatographic device for cleanup and concentration of TNT from "real field" samples in a manner that complies with both chemical and immunochemical residue analysis methods.
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Immunochemical approaches for purification and detection of TNT traces by antibodies entrapped in a sol-gel matrix
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Altstein, M., Department of Cell Biology and Neurosciences, University of California Riverside, Boyce Hall 5419, Riverside, CA 92521-0146, United States, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Bronshtein, A., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Glattstein, B., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Zeichner, A., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Tamiri, T., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Almog, J., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Immunochemical approaches for purification and detection of TNT traces by antibodies entrapped in a sol-gel matrix
A highly sensitive immunochemical method for immunoaffinity purification (IAP) and detection of trace amounts of TNT was developed on the basis of antibodies (Abs) in a ceramic matrix (sol-gel). The study resulted in: (i) a highly sensitive and reproducible TNT ELISA (I50 and I20 values of 0.4 ± 0.09 ppb and 0.12 ± 0.03 ppb, respectively, n = 12), which is highly specific to TNT; and (ii) successful entrapment of the Abs that bound free analyte from solution. Binding was found to be highly reproducible, dose dependent, and only slightly (1.2-1.8-fold) lower than that in solution. The entrapped Abs did not leach from the matrix and were tolerant of absolute ethanol, acetone, and acetonitrile. Bound analytes could be easily eluted from the sol-gel matrix at high recoveries. The sol-gel-based IAP method described above introduces a simple one-step procedure that has a high potential to serve as a suitable and convenient immunochromatographic device for cleanup and concentration of TNT from "real field" samples in a manner that complies with both chemical and immunochemical residue analysis methods.
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