אסיף מאגר המחקר החקלאי

TraceHaplotyper: Using direct sequencing to determine the phase of an indel followed by biallelic SNPs

Year:

2007

Source of publication :

Authors :

Volume :

43

Co-Authors:

Seroussi, Y., Technion-Israel Institute of Technology, Haifa, Israel
Seroussi, E., Volcani Center, Bet-Dagan, Israel, Agricultural Research Organization, Volcani Center, Institute of Animal Science, P.O.B 6, Bet Dagan 5020, Israel

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Abstract:

Sequencing of a diploid PCR product that is heterozygous for an indel mutation and a downstream single nucleotide polymorphism (SNP) allows determination of haplotype phase. Manual determination of phase from trace files of this type is tedious; therefore, we introduce the Perl script TraceHaplotyper, which works from the expected sequence to analyze a sequencing trace file for the sites that are informative for the genotyping and outputs the two underlying haplotypes.

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