Co-Authors:
Ketsa, S., Department of Horticulture, Fac. of Agric., Kasetsart University, Bangkok 10900, Thailand
Chidtragool, S., Yasothon Agric. and Technol. College, Amphur Muang, Yasothon 35000, Thailand
Klein, J.D., The Volcani Center, ARO, Bet Dagan 50250, Israel
Lurie, S., The Volcani Center, ARO, Bet Dagan 50250, Israel
Abstract:
Mango fruits (Mangifera indica L. cv. Nam Dokmai) were held for 3 days at 38°C and then transferred to 20°C. Fruits placed directly at 25°C after harvest reached their peak ethylene production after 4 days, while the heated fruit took 9 days (6 days after the end of the heat treatment). The 1-aminocyclopropane-1-carboxylic acid (ACC) content paralleled that of ethylene production in unheated fruits, with highest content occurring on day 4. In heated fruit, ACC content continued to increase for 8 days following the heat treatment. The 1-aminocyclopropane-1-carboxylic acid synthase (ACS) activity in unheated fruit was also highest on day 4. In heated fruit the activity was undetectable following heat treatment, but recovered partially during the ripening period. Initial ethylene production from discs with peel was 6-fold higher in unheated fruit than heated, although after 6 h of incubation the production from both was similar. Activity of 1-aminocyclopropane-carboxylic acid oxidase (ACO) in the discs also showed an 8-fold difference in initial activity in heated versus unheated discs with peel. This difference disappeared during incubation of the discs. In heated discs without peel, both ethylene and ACO activity remained lower than in discs with peel, and the differences between heated and unheated fruit discs were more pronounced. We conclude that the inhibition of ethylene production found during heat treatment is due to inhibition of both ACS and ACO. ACO recovers full activity following heat, while ACS activity only partially, but enough to allow the heated fruit to achieve an ethylene peak.