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Phytochemical Analysis
Lewinsohn, E., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Savage, T.J., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Gijzen, M., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States, Agriculture Canada Research Centre, London, Ontario, N6G 2V4, Canada
Croteau, R., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
A simple and rapid procedure for the simultaneous determination of monoterpenes and diterpenoid resin acids of conifer stem oleoresin is described; it involves tissue extraction with methyl tert‐butyl ether and methylation of the resin acids with CH2N2. The resin acid methyl esters and the monoterpenes are then analysed in a single gas chromatographic step. Enantiomer composition of the major monoterpene constituents is determined, after preliminary purification by thin layer chromatography, by capillary gas chromatography on a cyclodextrin column. The method is applicable to conifer species of high resin content (Iodgepole pine) and low resin content (grand fir). Copyright © 1993 John Wiley & Sons, Ltd
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Simultaneous analysis of monoterpenes and diterpenoids of conifer oleoresin
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Lewinsohn, E., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Savage, T.J., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Gijzen, M., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States, Agriculture Canada Research Centre, London, Ontario, N6G 2V4, Canada
Croteau, R., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Simultaneous analysis of monoterpenes and diterpenoids of conifer oleoresin
A simple and rapid procedure for the simultaneous determination of monoterpenes and diterpenoid resin acids of conifer stem oleoresin is described; it involves tissue extraction with methyl tert‐butyl ether and methylation of the resin acids with CH2N2. The resin acid methyl esters and the monoterpenes are then analysed in a single gas chromatographic step. Enantiomer composition of the major monoterpene constituents is determined, after preliminary purification by thin layer chromatography, by capillary gas chromatography on a cyclodextrin column. The method is applicable to conifer species of high resin content (Iodgepole pine) and low resin content (grand fir). Copyright © 1993 John Wiley & Sons, Ltd
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