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Simultaneous analysis of monoterpenes and diterpenoids of conifer oleoresin
Year:
1993
Source of publication :
Phytochemical Analysis
Authors :
Lewinsohn, Efraim
;
.
Volume :
4
Co-Authors:
Lewinsohn, E., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Savage, T.J., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Gijzen, M., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States, Agriculture Canada Research Centre, London, Ontario, N6G 2V4, Canada
Croteau, R., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Facilitators :
From page:
220
To page:
225
(
Total pages:
6
)
Abstract:
A simple and rapid procedure for the simultaneous determination of monoterpenes and diterpenoid resin acids of conifer stem oleoresin is described; it involves tissue extraction with methyl tert‐butyl ether and methylation of the resin acids with CH2N2. The resin acid methyl esters and the monoterpenes are then analysed in a single gas chromatographic step. Enantiomer composition of the major monoterpene constituents is determined, after preliminary purification by thin layer chromatography, by capillary gas chromatography on a cyclodextrin column. The method is applicable to conifer species of high resin content (Iodgepole pine) and low resin content (grand fir). Copyright © 1993 John Wiley & Sons, Ltd
Note:
Related Files :
Abies grandis
diterpenoid resin acids
grand fir
Iodgepole pine
Monoterpenes
oleoresin
Pinaceae
Pinus contorta
Show More
Related Content
More details
DOI :
10.1002/pca.2800040506
Article number:
Affiliations:
Database:
Scopus
Publication Type:
Review
;
.
Language:
English
Editors' remarks:
ID:
25894
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:18
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Scientific Publication
Simultaneous analysis of monoterpenes and diterpenoids of conifer oleoresin
4
Lewinsohn, E., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Savage, T.J., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Gijzen, M., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States, Agriculture Canada Research Centre, London, Ontario, N6G 2V4, Canada
Croteau, R., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164–6340, United States
Simultaneous analysis of monoterpenes and diterpenoids of conifer oleoresin
A simple and rapid procedure for the simultaneous determination of monoterpenes and diterpenoid resin acids of conifer stem oleoresin is described; it involves tissue extraction with methyl tert‐butyl ether and methylation of the resin acids with CH2N2. The resin acid methyl esters and the monoterpenes are then analysed in a single gas chromatographic step. Enantiomer composition of the major monoterpene constituents is determined, after preliminary purification by thin layer chromatography, by capillary gas chromatography on a cyclodextrin column. The method is applicable to conifer species of high resin content (Iodgepole pine) and low resin content (grand fir). Copyright © 1993 John Wiley & Sons, Ltd
Scientific Publication
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