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Morin, S., Dept. of Field Crops and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Ghanim, M., Dept. of Field Crops and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sobol, I., Dept. of Field Crops and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Czosnek, H., Dept. of Field Crops and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
We have previously suggested that a GroEL homolog produced by the whitefly Bemisia tabaci endosymbiotic bacteria interacts in the insect hemolymph with particles of Tomato yellow leaf curl virus from Israel (TYLCV-Is), ensuring the safe circulative transmission of the virus. We have now addressed the question of whether the nontransmissibility of Abutilon mosaic virus from Israel (AbMV-Is) is related to a lack of association between GroEL and the virus coat protein (CP). Translocation analysis has shown that, whereas TYLCV-Is DNA is conspicuous in the digestive tract, hemolymph, and salivary glands of B. tabaci 8 h after acquisition feeding started, AbMV-Is DNA was detected only in the insect digestive tract, even after 96 h. To determine whether AbMV-Is particles were rapidly degraded in the hemolymph as a result of their inability to interact with GroEL, we have isolated a GroEL gene from B. tabaci and used a yeast two-hybrid assay to compare binding of the CP of TYLCV-Is and AbMV-Is to the insect GroEL. The yeast assay showed that the CPs of the two viruses are able to bind efficiently to GroEL. We therefore suggest that, although GroEL-CP interaction in the hemolymph is a necessary condition for circulative transmission, the nontransmissibility of AbMV-Is is not the result of lack of binding to GroEL in the B. tabaci hemolymph, but most likely results from an inability to cross the gut/hemolymph barrier. (C) 2000 Academic Press.
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The GroEL protein of the whitefly Bemisia tabaci interacts with the coat protein of transmissible and nontransmissible begomoviruses in the yeast two-hybrid system
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Morin, S., Dept. of Field Crops and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Ghanim, M., Dept. of Field Crops and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sobol, I., Dept. of Field Crops and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Czosnek, H., Dept. of Field Crops and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
The GroEL protein of the whitefly Bemisia tabaci interacts with the coat protein of transmissible and nontransmissible begomoviruses in the yeast two-hybrid system
We have previously suggested that a GroEL homolog produced by the whitefly Bemisia tabaci endosymbiotic bacteria interacts in the insect hemolymph with particles of Tomato yellow leaf curl virus from Israel (TYLCV-Is), ensuring the safe circulative transmission of the virus. We have now addressed the question of whether the nontransmissibility of Abutilon mosaic virus from Israel (AbMV-Is) is related to a lack of association between GroEL and the virus coat protein (CP). Translocation analysis has shown that, whereas TYLCV-Is DNA is conspicuous in the digestive tract, hemolymph, and salivary glands of B. tabaci 8 h after acquisition feeding started, AbMV-Is DNA was detected only in the insect digestive tract, even after 96 h. To determine whether AbMV-Is particles were rapidly degraded in the hemolymph as a result of their inability to interact with GroEL, we have isolated a GroEL gene from B. tabaci and used a yeast two-hybrid assay to compare binding of the CP of TYLCV-Is and AbMV-Is to the insect GroEL. The yeast assay showed that the CPs of the two viruses are able to bind efficiently to GroEL. We therefore suggest that, although GroEL-CP interaction in the hemolymph is a necessary condition for circulative transmission, the nontransmissibility of AbMV-Is is not the result of lack of binding to GroEL in the B. tabaci hemolymph, but most likely results from an inability to cross the gut/hemolymph barrier. (C) 2000 Academic Press.
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