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Plant Science Letters
Butenko, R.G., Timiriazev Institute of Plant Physiology, the USSR Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russian Federation
Lipsky, A.Kh., Timiriazev Institute of Plant Physiology, the USSR Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russian Federation
Chernyak, N.D., Timiriazev Institute of Plant Physiology, the USSR Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russian Federation
Arya, H.C., Timiriazev Institute of Plant Physiology, the USSR Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russian Federation
The effect of initial pH of the culture medium (pHi) on growth, changes in pH of culture medium (pHc) and respiration of Dioscorea deltoidea cells was studied in cycle of batch cultivation. At pHi 3.50 the lag phase of growth and respiration was about 24 h. Within the pHi-range of 4.30-6.15 no lag phase was observed, growth and respiration being similar. At pHi-values of 2.00-2.50 and 8.00 the cell population died. At pH 3.50-6.15 the level of pHc was shifted to 4.6-4.7 by cells within 10-48 h. We suppose that the pH-regulation at pHi range of 3.50-6.15 by the cells to a definite value of the pHc (4.6-4.7) ensures their normal growthy cycle. © 1984.
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Changes in culture medium pH by cell suspension cultures of Dioscorea deltoidea
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Butenko, R.G., Timiriazev Institute of Plant Physiology, the USSR Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russian Federation
Lipsky, A.Kh., Timiriazev Institute of Plant Physiology, the USSR Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russian Federation
Chernyak, N.D., Timiriazev Institute of Plant Physiology, the USSR Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russian Federation
Arya, H.C., Timiriazev Institute of Plant Physiology, the USSR Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russian Federation
Changes in culture medium pH by cell suspension cultures of Dioscorea deltoidea
The effect of initial pH of the culture medium (pHi) on growth, changes in pH of culture medium (pHc) and respiration of Dioscorea deltoidea cells was studied in cycle of batch cultivation. At pHi 3.50 the lag phase of growth and respiration was about 24 h. Within the pHi-range of 4.30-6.15 no lag phase was observed, growth and respiration being similar. At pHi-values of 2.00-2.50 and 8.00 the cell population died. At pH 3.50-6.15 the level of pHc was shifted to 4.6-4.7 by cells within 10-48 h. We suppose that the pH-regulation at pHi range of 3.50-6.15 by the cells to a definite value of the pHc (4.6-4.7) ensures their normal growthy cycle. © 1984.
Scientific Publication
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