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Meng, B., Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON N1G2W1, Canada
Venkataraman, S., Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON N1G2W1, Canada
Li, C., Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON N1G2W1, Canada
Wang, W., Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON N1G2W1, Canada, Molecular Diagnostics Program, College o fHealth Professions, Ferris State University, BigRapids, MI, United States
Dayan-Glick, C., The Plant Pathology Department-The Virology Unit, Plant Protection Institute, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Mawassi, M., The Plant Pathology Department-The Virology Unit, Plant Protection Institute, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Grapevine rupestris stem pitting-associated virus (GRSPaV, genus Foveavirus, family Betaflexiviridae) is one of the most prevalent viruses in grapevines and is associated with three distinct diseases: rupestris stem pitting, vein necrosis and Syrah decline. Little is known about the biology and pathological properties of GRSPaV. In this work, we engineered a full-length infectious cDNA clone for GRSPaV and a GFP-tagged variant, both under the transcriptional control of Cauliflower mosaic virus 35. S promoter. We demonstrated that these cDNA clones were infectious in grapevines and Nicotiana benthamiana through fluorescence microscopy, RT-PCR, Western blotting and immuno electron microscopy. Interestingly, GRSPaV does not cause systemic infection in four of the most commonly used herbaceous plants, even in the presence of the movement proteins of two other viruses which are known to complement numerous movement-defective viruses. These infectious clones are the first of members of Foveavirus which would allow further investigations into mechanisms governing different aspects of replication for GRSPaV and perhaps related viruses. © 2012 Elsevier Inc.
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Construction and biological activities of the first infectious cDNA clones of the genus Foveavirus
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Meng, B., Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON N1G2W1, Canada
Venkataraman, S., Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON N1G2W1, Canada
Li, C., Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON N1G2W1, Canada
Wang, W., Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, ON N1G2W1, Canada, Molecular Diagnostics Program, College o fHealth Professions, Ferris State University, BigRapids, MI, United States
Dayan-Glick, C., The Plant Pathology Department-The Virology Unit, Plant Protection Institute, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Mawassi, M., The Plant Pathology Department-The Virology Unit, Plant Protection Institute, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Construction and biological activities of the first infectious cDNA clones of the genus Foveavirus
Grapevine rupestris stem pitting-associated virus (GRSPaV, genus Foveavirus, family Betaflexiviridae) is one of the most prevalent viruses in grapevines and is associated with three distinct diseases: rupestris stem pitting, vein necrosis and Syrah decline. Little is known about the biology and pathological properties of GRSPaV. In this work, we engineered a full-length infectious cDNA clone for GRSPaV and a GFP-tagged variant, both under the transcriptional control of Cauliflower mosaic virus 35. S promoter. We demonstrated that these cDNA clones were infectious in grapevines and Nicotiana benthamiana through fluorescence microscopy, RT-PCR, Western blotting and immuno electron microscopy. Interestingly, GRSPaV does not cause systemic infection in four of the most commonly used herbaceous plants, even in the presence of the movement proteins of two other viruses which are known to complement numerous movement-defective viruses. These infectious clones are the first of members of Foveavirus which would allow further investigations into mechanisms governing different aspects of replication for GRSPaV and perhaps related viruses. © 2012 Elsevier Inc.
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