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Mechanism of uptake of sulfonated metallophthalocyanines by cultured mammalian cells
Year:
1987
Source of publication :
Cancer Letters
Authors :
Rosenthal, Ionel
;
.
Volume :
38
Co-Authors:
Ben-Hur, E., Nuclear Research Center-Negev, P.O. Box 9001, Beer-Sheva, 84190, Israel
Siwecki, J.A., North Carolina State University, School of Veterinary Medicine, Raleigh, NC 27606, United States
Newman, H.C., North Carolina State University, School of Veterinary Medicine, Raleigh, NC 27606, United States
Crane, S.W., North Carolina State University, School of Veterinary Medicine, Raleigh, NC 27606, United States
Rosenthal, I., A.R.O. Department of Food Science, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Facilitators :
From page:
215
To page:
222
(
Total pages:
8
)
Abstract:
The uptake of several metallophthalocyanine tetrasulfonates by cultured Chinese hamster cells was studied. Uranyl- and chloraluminium were taken up at the highest rate followed by Ni-, Zn-, Cu-, Co- and dichlorosiliconphthalocyanine tetrasulfonate. The uptake from the growth medium containing 10% serum in which only 15% of the dye is not bound to serum proteins was 5-18-fold slower than in the absence of serum, suggesting that most of the uptake is of free dye. Using gel filtration to separate tightly protein-bound dye from the free dye, binding curves were constructed that varied in slope and saturation values for the different compounds. At saturation, the number of dye molecules bound per serum albumin molecule varied from 1 for vanadyl- and cobalt-, to 4 for uranyl-phthalocyanine tetrasulfonate. Absorption spectra of the various phthalocyanines indicated that under physiological conditions, all dyes, with the exception of chloroaluminum- and dichlorosilicon-phthalocyanine tetrasulfonate, were aggregated. The rate of uptake was unrelated to the state of aggregation. The rate of uptake was temperature dependent at intervals longer than 1 h. At shorter times, very little temperature dependence was observed. These results suggest that the uptake process takes place in two steps. The first step is passive, involving binding of metallophthalocyanine tetrasulfonate to a receptor on the cell membrane, while the second one is active and involves internalization of the bound dye. © 1987.
Note:
Related Files :
Animal
animal cell
radiosensitizing agent
Serum Albumin, Bovine
temperature
unclassified drug
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More details
DOI :
10.1016/0304-3835(87)90217-5
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26055
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:19
Scientific Publication
Mechanism of uptake of sulfonated metallophthalocyanines by cultured mammalian cells
38
Ben-Hur, E., Nuclear Research Center-Negev, P.O. Box 9001, Beer-Sheva, 84190, Israel
Siwecki, J.A., North Carolina State University, School of Veterinary Medicine, Raleigh, NC 27606, United States
Newman, H.C., North Carolina State University, School of Veterinary Medicine, Raleigh, NC 27606, United States
Crane, S.W., North Carolina State University, School of Veterinary Medicine, Raleigh, NC 27606, United States
Rosenthal, I., A.R.O. Department of Food Science, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Mechanism of uptake of sulfonated metallophthalocyanines by cultured mammalian cells
The uptake of several metallophthalocyanine tetrasulfonates by cultured Chinese hamster cells was studied. Uranyl- and chloraluminium were taken up at the highest rate followed by Ni-, Zn-, Cu-, Co- and dichlorosiliconphthalocyanine tetrasulfonate. The uptake from the growth medium containing 10% serum in which only 15% of the dye is not bound to serum proteins was 5-18-fold slower than in the absence of serum, suggesting that most of the uptake is of free dye. Using gel filtration to separate tightly protein-bound dye from the free dye, binding curves were constructed that varied in slope and saturation values for the different compounds. At saturation, the number of dye molecules bound per serum albumin molecule varied from 1 for vanadyl- and cobalt-, to 4 for uranyl-phthalocyanine tetrasulfonate. Absorption spectra of the various phthalocyanines indicated that under physiological conditions, all dyes, with the exception of chloroaluminum- and dichlorosilicon-phthalocyanine tetrasulfonate, were aggregated. The rate of uptake was unrelated to the state of aggregation. The rate of uptake was temperature dependent at intervals longer than 1 h. At shorter times, very little temperature dependence was observed. These results suggest that the uptake process takes place in two steps. The first step is passive, involving binding of metallophthalocyanine tetrasulfonate to a receptor on the cell membrane, while the second one is active and involves internalization of the bound dye. © 1987.
Scientific Publication
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