אסיף מאגר המחקר החקלאי

Morphological, genetic, and pathogenic characterization of colletotrichum acutatum, the cause of anthracnose of almond in australia

Year:

2009

Source of publication :

Authors :

Volume :

99

Co-Authors:

McKay, S.F., University of Adelaide, School of Agriculture Food and Wine, PMB 1 Glen Osmond, SA, 5064, Australia
Freeman, S., Department of Plant Pathology
Minz, D., Institute for Soil, Water and Environmental Sciences ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Maymon, M., Department of Plant Pathology
Sedgley, M., University of New England, Faculty of the Arts and Sciences, Armidale, NSW, 2351, Australia
Collins, G.C., University of Adelaide, School of Agriculture Food and Wine, PMB 1 Glen Osmond, SA, 5064, Australia
Scott, E.S., University of Adelaide, School of Agriculture Food and Wine, PMB 1 Glen Osmond, SA, 5064, Australia

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Abstract:

Abstract Almond anthracnose was reported for the first time in Australia in 1998 and has since been observed in all of the major almond-growing regions. The organism causing anthracnose was confirmed as Colletot- richum acutatum using taxon-specific polymerase chain reaction (PCR). Three main morphotypes of C. acutatum from almond in Australia were identified (namely, pink, orange, and cream colony color) and the optimum temperature for mycelial growth of representative isolates was 25°C. Australian isolates of C. acutatum were more similar morphologically to the pink subpopulation of C. acutatum from California than to the gray Californian subpopulation and the isolates of Colletotrichum from Israel. Inter-simple-sequence-repeat (ISSR) PCR analysis revealed that the majority of Australian isolates shared an identical banding pattern whereas Australian isolates of C. acutatum from almond were distinct from isolates of the pink and gray subpopulations of C. acutatum from almond in California and of Colletotrichum spp. from almond in Israel. Sequence analysis of the internally transcribed spacer (ITS1-2) ribosomal DNA region of representative isolates differed from the results of ISSR- PCR in that polymorphisms were revealed among isolates, indicating that some genetic variation may be present. Pathogenicity experiments on detached leaves and fruit revealed pathogenic variation among representative isolates of C. acutatum from almond in Australia, California, and Israel; however, all isolates tested caused disease. Distinct subgroups among Australian isolates of C. acutatum from almond were not supported on the basis of morphology, mycelial growth rates, ISSR-PCR, and pathogenicity. © 2009 The American Phytopathological Society.

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