Co-Authors:
Chkhikvishvili, I., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Ave. 0177, Tbilisi, Georgia
Sanikidze, T., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Ave. 0177, Tbilisi, Georgia
Gogia, N., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Ave. 0177, Tbilisi, Georgia
McHedlishvili, T., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Ave. 0177, Tbilisi, Georgia
Enukidze, M., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Ave. 0177, Tbilisi, Georgia
Machavariani, M., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Ave. 0177, Tbilisi, Georgia
Vinokur, Y., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Rodov, V., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Abstract:
Summer savory (Satureja hortensis L., Lamiaceae) is used in several regions of the world as a spice and folk medicine. Anti-inflammatory and cytoprotective effects of S. hortensis and of its rosmarinic acid-rich phenolic fraction have been demonstrated in animal trials. However, previous studies of rosmarinic acid in cell models have yielded controversial results. In this study, we investigated the effects of summer savory extracts on H2O 2-challenged human lymphoblastoid Jurkat T cells. LC-MS analysis confirmed the presence of rosmarinic acid and flavonoids such as hesperidin and naringin in the phenolic fraction. Adding 25 or 50 μM of H2O 2 to the cell culture caused oxidative stress, manifested as generation of superoxide and peroxyl radicals, reduced cell viability, G0/G1 arrest, and enhanced apoptosis. This stress was significantly alleviated by the ethanolic and aqueous extracts of S. hortensis and by the partially purified rosmarinic acid fraction. The application of an aqueous S. hortensis extract doubled the activity of catalase and superoxide dismutase in the cells. The production of IL-2 and IL-10 interleukins was stimulated by H2O 2 and was further enhanced by the addition of the S. hortensis extract or rosmarinic acid fraction. The H2O2-challenged Jurkat cells may serve as a model for investigating cellular mechanisms of cytoprotective phytonutrient effects. © 2013 Irakli Chkhikvishvili et al.
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