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Immunogenetics
Seroussi, E., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Beit Dagan 50250, Israel
Klompus, S., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Beit Dagan 50250, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Jerusalem, Israel
Silanikove, M., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Beit Dagan 50250, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Jerusalem, Israel
Krifucks, O., National Mastitis Reference Center, Kimron Veterinary Institute, Beit Dagan, Israel
Shapiro, F., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Beit Dagan 50250, Israel
Gertler, A., Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Jerusalem, Israel
Leitner, G., National Mastitis Reference Center, Kimron Veterinary Institute, Beit Dagan, Israel
The recent burst of duplication and divergence of the bovine PLA2G2D genes is considered typical of immune response genes, and it was recently shown that PLA2G2D is abundantly expressed in mouse leukocytes and acts as an immunosuppressive phospholipase. Analysis of 1,143 Holstein bulls indicated that the four common haplotypes spanning PLA2G2D display copy number variation ranging from 1 to 4 per haploid genome. Association of the fourth haplotype with negative total merit remained significant (P < 0.002) when corrected for population relatedness. We compared the lipase and bactericidal activities of bovine pancreatic PLA2G1B with human PLA2G2A and G2D and bovine PLA2G2D1 and G2D4 proteins, which had been subcloned, expressed, and refolded by us, and the impact of point mutations in the calcium binding site was investigated. All tested phospholipases were ineffective bactericides of Escherichia coli isolated from bovine mastitis. However, in lactating mice treated with E. coli or lipopolysaccharide (LPS), intramammary injection of bovine PLA2G1B relieved visual and histological inflammation and reduced blood levels of infiltrating lactose. Further studies are warranted to determine whether the observed anti-inflammatory effect involves competitive binding of the receptor Pla2r1 which may mimic the LPS resistance effect in Pla2r1-deficient mice. © 2013 Springer-Verlag Berlin Heidelberg.
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Nonbactericidal secreted phospholipase A2s are potential anti-inflammatory factors in the mammary gland
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Seroussi, E., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Beit Dagan 50250, Israel
Klompus, S., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Beit Dagan 50250, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Jerusalem, Israel
Silanikove, M., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Beit Dagan 50250, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Jerusalem, Israel
Krifucks, O., National Mastitis Reference Center, Kimron Veterinary Institute, Beit Dagan, Israel
Shapiro, F., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Beit Dagan 50250, Israel
Gertler, A., Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Jerusalem, Israel
Leitner, G., National Mastitis Reference Center, Kimron Veterinary Institute, Beit Dagan, Israel
Nonbactericidal secreted phospholipase A2s are potential anti-inflammatory factors in the mammary gland
The recent burst of duplication and divergence of the bovine PLA2G2D genes is considered typical of immune response genes, and it was recently shown that PLA2G2D is abundantly expressed in mouse leukocytes and acts as an immunosuppressive phospholipase. Analysis of 1,143 Holstein bulls indicated that the four common haplotypes spanning PLA2G2D display copy number variation ranging from 1 to 4 per haploid genome. Association of the fourth haplotype with negative total merit remained significant (P < 0.002) when corrected for population relatedness. We compared the lipase and bactericidal activities of bovine pancreatic PLA2G1B with human PLA2G2A and G2D and bovine PLA2G2D1 and G2D4 proteins, which had been subcloned, expressed, and refolded by us, and the impact of point mutations in the calcium binding site was investigated. All tested phospholipases were ineffective bactericides of Escherichia coli isolated from bovine mastitis. However, in lactating mice treated with E. coli or lipopolysaccharide (LPS), intramammary injection of bovine PLA2G1B relieved visual and histological inflammation and reduced blood levels of infiltrating lactose. Further studies are warranted to determine whether the observed anti-inflammatory effect involves competitive binding of the receptor Pla2r1 which may mimic the LPS resistance effect in Pla2r1-deficient mice. © 2013 Springer-Verlag Berlin Heidelberg.
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