אסיף מאגר המחקר החקלאי

Ribosome associated protein(s) specifically bind(s) to the upstream activator sequence of the E. coli rrnA P1 promoter

Year:

1989

Source of publication :

Authors :

Volume :

17

Co-Authors:

Nachaliel, N., Department of Cellular Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel
Melnick, J., Department of Cellular Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel
Gafny, R., Department of Cellular Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel
Glaser, G., Department of Cellular Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel

Facilitators :

Link :

Abstract:

A sequence located upstream to the E. coli rrnA P1 promoter is required for optimal promoter activity. Deletion of this sequence reduces in vivo transcription by 90%. Substitution of this upstream activating sequence with the unrelated bent DNA sequence of the kinetoplast of Crithidia fasciculata, restores in vivo expression to high levels. Cellular proteins which are present only in exponentially growing cells bind specifically to intact rrnP1, bet do not bind to the promoter missing the upstream asivating sequence. These proteins are associated with the 30S ribosomal subunits but can be washed off with concentrated salt. The correlation between the binding activity and cell growth rate suggests a role for these proteins in the transcriptional control of rRNA synthesis. © 1989 IRL Press.

Note:

Related Files :

Related Content

More details

You may also be interested in