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Plant Molecular Biology Reporter
Caiqing, M., Department of Natural Resource, Institute of Field and Garden Crops, Volcani Center, Bet-Dagan, 50250, Israel, Microbiology Department, Ctrl. China (Huazhong) Agric. Univ., Wuhan, 430070, China, Minerva Ctr. Mar. Invert. I., Israel Natl. Inst. of Oceanography, Tel-Shikmona, P.O. Box 8030, Haifa 31080, Israel
Kapulnik, Y., Department of Natural Resource, Institute of Field and Garden Crops, Volcani Center, Bet-Dagan, 50250, Israel
A novel strategy based on protein western blotting and "microcosm" principles has been developed to study gene expression in transgenic plant roots. Primary polyclonal antibodies, raised against streptavidin protein purified from Streptomyces avidinii, were used to localize streptavidin protein expressed in a transgenic tobacco seedling root system in situ. Streptavidin gene expression was detected in tobacco seedling roots, especially on the root tips. This strategy can be used as a model to study transgenic plant root system expression in situ.
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In Situ Western Blot: A Novel Strategy to Study Gene Expression in Transgenic Plant Roots
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Caiqing, M., Department of Natural Resource, Institute of Field and Garden Crops, Volcani Center, Bet-Dagan, 50250, Israel, Microbiology Department, Ctrl. China (Huazhong) Agric. Univ., Wuhan, 430070, China, Minerva Ctr. Mar. Invert. I., Israel Natl. Inst. of Oceanography, Tel-Shikmona, P.O. Box 8030, Haifa 31080, Israel
Kapulnik, Y., Department of Natural Resource, Institute of Field and Garden Crops, Volcani Center, Bet-Dagan, 50250, Israel
In Situ Western Blot: A Novel Strategy to Study Gene Expression in Transgenic Plant Roots
A novel strategy based on protein western blotting and "microcosm" principles has been developed to study gene expression in transgenic plant roots. Primary polyclonal antibodies, raised against streptavidin protein purified from Streptomyces avidinii, were used to localize streptavidin protein expressed in a transgenic tobacco seedling root system in situ. Streptavidin gene expression was detected in tobacco seedling roots, especially on the root tips. This strategy can be used as a model to study transgenic plant root system expression in situ.
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