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In Situ Western Blot: A Novel Strategy to Study Gene Expression in Transgenic Plant Roots
Year:
2000
Source of publication :
Plant Molecular Biology Reporter
Authors :
Kapulnik, Yoram
;
.
Volume :
18
Co-Authors:
Caiqing, M., Department of Natural Resource, Institute of Field and Garden Crops, Volcani Center, Bet-Dagan, 50250, Israel, Microbiology Department, Ctrl. China (Huazhong) Agric. Univ., Wuhan, 430070, China, Minerva Ctr. Mar. Invert. I., Israel Natl. Inst. of Oceanography, Tel-Shikmona, P.O. Box 8030, Haifa 31080, Israel
Kapulnik, Y., Department of Natural Resource, Institute of Field and Garden Crops, Volcani Center, Bet-Dagan, 50250, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
A novel strategy based on protein western blotting and "microcosm" principles has been developed to study gene expression in transgenic plant roots. Primary polyclonal antibodies, raised against streptavidin protein purified from Streptomyces avidinii, were used to localize streptavidin protein expressed in a transgenic tobacco seedling root system in situ. Streptavidin gene expression was detected in tobacco seedling roots, especially on the root tips. This strategy can be used as a model to study transgenic plant root system expression in situ.
Note:
Related Files :
In situ western blot
Root expression
Streptavidin gene
transgenic plant
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26218
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:21
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Scientific Publication
In Situ Western Blot: A Novel Strategy to Study Gene Expression in Transgenic Plant Roots
18
Caiqing, M., Department of Natural Resource, Institute of Field and Garden Crops, Volcani Center, Bet-Dagan, 50250, Israel, Microbiology Department, Ctrl. China (Huazhong) Agric. Univ., Wuhan, 430070, China, Minerva Ctr. Mar. Invert. I., Israel Natl. Inst. of Oceanography, Tel-Shikmona, P.O. Box 8030, Haifa 31080, Israel
Kapulnik, Y., Department of Natural Resource, Institute of Field and Garden Crops, Volcani Center, Bet-Dagan, 50250, Israel
In Situ Western Blot: A Novel Strategy to Study Gene Expression in Transgenic Plant Roots
A novel strategy based on protein western blotting and "microcosm" principles has been developed to study gene expression in transgenic plant roots. Primary polyclonal antibodies, raised against streptavidin protein purified from Streptomyces avidinii, were used to localize streptavidin protein expressed in a transgenic tobacco seedling root system in situ. Streptavidin gene expression was detected in tobacco seedling roots, especially on the root tips. This strategy can be used as a model to study transgenic plant root system expression in situ.
Scientific Publication
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