אסיף מאגר המחקר החקלאי

Action of pheromone biosynthesis activating neuropeptide on In vitro pheromone glands of Heliothis armigera females

Year:

1990

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Volume :

36

Co-Authors:

Rafaeli, A., Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76-100, Israel
Soroker, V., Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76-100, Israel
Kamensky, B., Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76-100, Israel
Raina, A.K., Insect Chemical Ecology Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States

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Abstract:

Pheromone glands of Heliothis armigera were stimulated in vitro to incorporate 14C from the radioactive precursor sodium acetate, in the presence of synthetic pheromone biosynthesis activating neuropeptide (PBAN). When hexane extracts of the radioactive products were analysed by TLC and HPLC the radioactivity corresponded in retention time to the main pheromone component (Z)-11-hexadecenal. Maximal stimulation, as depicted by TLC analysis, was observed after 4 h of incubation. The PBAN response was shown to be dose dependent, maximal levels, as analysed by TLC, were obtained at a concentration of 5 pmol/gland. This response was shown to be mediated by cAMP. © 1990.

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