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Journal of Applied Entomology
Nestel, D., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Beit Dagan, Israel
Nemny-Lavy, E., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel
Zilberg, L., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel
Weiss, M., Plant Protect. and Inspection Serv., Ministry of Agriculture, Beit-Dagan, Israel
Akiva, R., Institute of Biological Control, Citrus Marketing Board of Israel, Beit-Dagan, Israel
Gazit, Y., Institute of Biological Control, Citrus Marketing Board of Israel, Beit-Dagan, Israel
A bioassay to investigate quantitative phagostimulation and ingestion physiology of baits on individual fruit flies is presented. The study was undertaken using two fruit fly species: the Mediterranean fruit fly (Ceratitis capitata), a cosmopolitan insect pest, and the Ethiopian fruit fly (Dacus ciliatus), a quarantine insect in Israel. Our model bait suspension included spinosad as the toxic agent, and 1% yeast hydrolysate with 10% sucrose as phagostimulant. A preliminary toxicology study showed that the two fruit flies are highly sensitive to low concentrations of spinosad baited with this phagostimulant. The maximum concentration needed to kill 90% of the female flies was 4.2 and 8.5 p.p.m. for C. capitata and D. ciliatus, respectively. The bioassay was able to detect the ingestion of low volumes (e.g. 1 μl) of tested solutions. The bioassay was also able to detect differences in intake of different concentrations of spinosad solutions and relate ingestion to fruit fly mortality. Additionally, the bioassay was sensitive enough to highlight differences in intake related to the physiological status of the fruit fly and fly species. The bioassay can also be used to follow ingestion kinetics of baits. We expect that this bioassay will contribute in the exploration of more efficient bait systems for fruit flies.
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The fruit fly PUB: A phagostimulation unit bioassay system to quantitatively measure ingestion of baits by individual flies
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Nestel, D., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Beit Dagan, Israel
Nemny-Lavy, E., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel
Zilberg, L., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel
Weiss, M., Plant Protect. and Inspection Serv., Ministry of Agriculture, Beit-Dagan, Israel
Akiva, R., Institute of Biological Control, Citrus Marketing Board of Israel, Beit-Dagan, Israel
Gazit, Y., Institute of Biological Control, Citrus Marketing Board of Israel, Beit-Dagan, Israel
The fruit fly PUB: A phagostimulation unit bioassay system to quantitatively measure ingestion of baits by individual flies
A bioassay to investigate quantitative phagostimulation and ingestion physiology of baits on individual fruit flies is presented. The study was undertaken using two fruit fly species: the Mediterranean fruit fly (Ceratitis capitata), a cosmopolitan insect pest, and the Ethiopian fruit fly (Dacus ciliatus), a quarantine insect in Israel. Our model bait suspension included spinosad as the toxic agent, and 1% yeast hydrolysate with 10% sucrose as phagostimulant. A preliminary toxicology study showed that the two fruit flies are highly sensitive to low concentrations of spinosad baited with this phagostimulant. The maximum concentration needed to kill 90% of the female flies was 4.2 and 8.5 p.p.m. for C. capitata and D. ciliatus, respectively. The bioassay was able to detect the ingestion of low volumes (e.g. 1 μl) of tested solutions. The bioassay was also able to detect differences in intake of different concentrations of spinosad solutions and relate ingestion to fruit fly mortality. Additionally, the bioassay was sensitive enough to highlight differences in intake related to the physiological status of the fruit fly and fly species. The bioassay can also be used to follow ingestion kinetics of baits. We expect that this bioassay will contribute in the exploration of more efficient bait systems for fruit flies.
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