Specific combinations of human serum albumin introns direct high level expression of albumin in transfected COS cells and in the milk of transgenic mice

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Specific combinations of human serum albumin introns direct high level expression of albumin in transfected COS cells and in the milk of transgenic mice

Year: 

1994

Source of publication :

Transgenic Research

Authors :

Barash, Itamar

;

Shani, Moshe

.

Volume :

Co-Authors: 

Hurwitz, D.R., Rhône-Poulenc Rorer Central Research, 500 Arcola Road, Collegeville, 19426, PA, United States
Nathan, M., Rhône-Poulenc Rorer Central Research, 500 Arcola Road, Collegeville, 19426, PA, United States
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Ilan, N., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Shani, M., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel

From page: 

365

To page: 

375

(

Total pages: 

11

)

Link :

Specific combinations of human serum albumin introns direct high level expression of albumin in transfected COS cells and in the milk of transgenic mice

Abstract: 

A new series of expression vectors, each comprised of the β-lactoglobulin (BLG) promoter driving one of a variety of human serum albumin (HSA) minigenes or the entire gene, were evaluated for their ability to direct expression of HSA in vitro in COS tissue culture cells and into the milk of transgenic mice. Vectors directed a hierarchy of expression levels in vitro, dependent upon the specific complement of HSA introns included. HSA introns acted in a synergistic manner. In addition, minigenes comprised of specific subsets of introns were more efficacious than the entire HSA gene with all of its introns. Transgenic mice expressed as much as 10 mg ml-1 of HSA in their milk. Vectors comprised of specific intron subsets directed levels at 1 mg ml-1 or greater in the milk of 20% of generated transgenics. A statistical correlation between the expression level trend in vitro with the trend of expression in vivo (% which express) at detectable levels (p=0.0015) and at the level of greater than 0.1 mg ml-1 (p=0.0156) was demonstrated. A weak correlation existed (p=0.0526) at in vivo levels of 1 mg ml-1 or greater. These new vectors are expected to direct the production of high levels of HSA in the milk of a large percentage of generated transgenic dairy animals. © 1994 Chapman & Hall.