Plant Journal
Gonda, I., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Lev, S., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Bar, E., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Sikron, N., Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Portnoy, V., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Davidovich-Rikanati, R., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Burger, J., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Schaffer, A.A., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, PO Box 6, Bet, Dagan 76100, Israel
Tadmor, Y., Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Giovannonni, J.J., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, United States, Agricultural Research Service, US Department of Agriculture, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, United States
Huang, M., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, United States
Fei, Z., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, United States, Agricultural Research Service, US Department of Agriculture, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, United States
Katzir, N., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Fait, A., Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Lewinsohn, E., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Sulfur-containing aroma volatiles are important contributors to the distinctive aroma of melon and other fruits. Melon cultivars and accessions differ in the content of sulfur-containing and other volatiles. l-methionine has been postulated to serve as a precursor of these volatiles. Incubation of melon fruit cubes with 13C- and 2H-labeled l-methionine revealed two distinct catabolic routes into volatiles. One route apparently involves the action of an l-methionine aminotransferase and preserves the main carbon skeleton of l-methionine. The second route apparently involves the action of an l-methionine-γ-lyase activity, releasing methanethiol, a backbone for formation of thiol-derived aroma volatiles. Exogenous l-methionine also generated non-sulfur volatiles by further metabolism of α-ketobutyrate, a product of l-methionine-γ-lyase activity. α-Ketobutyrate was further metabolized into l-isoleucine and other important melon volatiles, including non-sulfur branched and straight-chain esters. Cell-free extracts derived from ripe melon fruit exhibited l-methionine-γ-lyase enzymatic activity. A melon gene (CmMGL) ectopically expressed in Escherichia coli, was shown to encode a protein possessing l-methionine-γ-lyase enzymatic activity. Expression of CmMGL was relatively low in early stages of melon fruit development, but increased in the flesh of ripe fruits, depending on the cultivar tested. Moreover, the levels of expression of CmMGL in recombinant inbred lines co-segregated with the levels of sulfur-containing aroma volatiles enriched with +1 m/z unit and postulated to be produced via this route. Our results indicate that l-methionine is a precursor of both sulfur and non-sulfur aroma volatiles in melon fruit. © 2013 The Authors The Plant Journal © 2013 Blackwell Publishing Ltd.
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Catabolism of l -methionine in the formation of sulfur and other volatiles in melon (Cucumis melo L.) fruit
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Gonda, I., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Lev, S., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Bar, E., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Sikron, N., Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Portnoy, V., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Davidovich-Rikanati, R., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Burger, J., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Schaffer, A.A., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, PO Box 6, Bet, Dagan 76100, Israel
Tadmor, Y., Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Giovannonni, J.J., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, United States, Agricultural Research Service, US Department of Agriculture, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, United States
Huang, M., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, United States
Fei, Z., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, United States, Agricultural Research Service, US Department of Agriculture, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, United States
Katzir, N., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Fait, A., Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Lewinsohn, E., Institute of Plant Sciences, Newe ya'Ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel
Catabolism of l -methionine in the formation of sulfur and other volatiles in melon (Cucumis melo L.) fruit
Sulfur-containing aroma volatiles are important contributors to the distinctive aroma of melon and other fruits. Melon cultivars and accessions differ in the content of sulfur-containing and other volatiles. l-methionine has been postulated to serve as a precursor of these volatiles. Incubation of melon fruit cubes with 13C- and 2H-labeled l-methionine revealed two distinct catabolic routes into volatiles. One route apparently involves the action of an l-methionine aminotransferase and preserves the main carbon skeleton of l-methionine. The second route apparently involves the action of an l-methionine-γ-lyase activity, releasing methanethiol, a backbone for formation of thiol-derived aroma volatiles. Exogenous l-methionine also generated non-sulfur volatiles by further metabolism of α-ketobutyrate, a product of l-methionine-γ-lyase activity. α-Ketobutyrate was further metabolized into l-isoleucine and other important melon volatiles, including non-sulfur branched and straight-chain esters. Cell-free extracts derived from ripe melon fruit exhibited l-methionine-γ-lyase enzymatic activity. A melon gene (CmMGL) ectopically expressed in Escherichia coli, was shown to encode a protein possessing l-methionine-γ-lyase enzymatic activity. Expression of CmMGL was relatively low in early stages of melon fruit development, but increased in the flesh of ripe fruits, depending on the cultivar tested. Moreover, the levels of expression of CmMGL in recombinant inbred lines co-segregated with the levels of sulfur-containing aroma volatiles enriched with +1 m/z unit and postulated to be produced via this route. Our results indicate that l-methionine is a precursor of both sulfur and non-sulfur aroma volatiles in melon fruit. © 2013 The Authors The Plant Journal © 2013 Blackwell Publishing Ltd.
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