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Molecular diagnosis of mango malformation disease and phylogeny of Fusarium mangiferae
Year:
2012
Source of publication :
Phytoparasitica
Authors :
Biton, Iris
;
.
Freeman, Stanley
;
.
Lavi, Uri
;
.
Maymon, Marcel
;
.
Newman, Zvi
;
.
Paz, Tali
;
.
Saada, David
;
.
Volume :
40
Co-Authors:
Newman, Z., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Biton, I., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Sa'ada, D., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Paz, T., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Maymon, M., Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Lavi, U., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
287
To page:
297
(
Total pages:
11
)
Abstract:
Mango malformation disease (MMD) caused by various Fusaria, including the fungus Fusarium mangiferae, is difficult to diagnose and cannot be controlled effectively. In this study a PCR diagnostic tool was developed for detection of F. mangiferae by generating primers which flank fungus-specific sequences. The assay is suitable for both in-vitro and in-vivo tests, and is capable of detecting 10 pg of the fungal DNA. Relatively high genetic variation was discovered between various isolates of the pathogen based on amplified fragment length polymorphism (AFLP) analyses. In addition, some isolates of F. mangiferae (including the accession numbers FMS-123 and NRRL 25226), were found to be quite distinct from most F. mangiferae accessions. Likewise, representative MMD-associated Fusaria from the Asian and American clades of the Gibberella fujikuroi species complex were further differentiated by AFLP analyses. © 2012 Springer Science + Business Media B.V.
Note:
Related Files :
fungi
Fusaria
Fusarium
Fusarium mangiferae
Gibberella fujikuroi species complex (GFSC)
Mangifera indica
Show More
Related Content
More details
DOI :
10.1007/s12600-012-0224-6
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26295
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:21
You may also be interested in
Scientific Publication
Molecular diagnosis of mango malformation disease and phylogeny of Fusarium mangiferae
40
Newman, Z., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Biton, I., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Sa'ada, D., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Paz, T., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Maymon, M., Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Lavi, U., Department of Fruit Trees, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 50250, Israel
Molecular diagnosis of mango malformation disease and phylogeny of Fusarium mangiferae
Mango malformation disease (MMD) caused by various Fusaria, including the fungus Fusarium mangiferae, is difficult to diagnose and cannot be controlled effectively. In this study a PCR diagnostic tool was developed for detection of F. mangiferae by generating primers which flank fungus-specific sequences. The assay is suitable for both in-vitro and in-vivo tests, and is capable of detecting 10 pg of the fungal DNA. Relatively high genetic variation was discovered between various isolates of the pathogen based on amplified fragment length polymorphism (AFLP) analyses. In addition, some isolates of F. mangiferae (including the accession numbers FMS-123 and NRRL 25226), were found to be quite distinct from most F. mangiferae accessions. Likewise, representative MMD-associated Fusaria from the Asian and American clades of the Gibberella fujikuroi species complex were further differentiated by AFLP analyses. © 2012 Springer Science + Business Media B.V.
Scientific Publication
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