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Theoretical and Applied Genetics
Sherman, A., Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Eshed, R., Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Harel-Beja, R., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Tzuri, G., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Portnoy, V., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Cohen, S., Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Rubinstein, M., Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Schaffer, A.A., Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Burger, J., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Katzir, N., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Ophir, R., Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
The availability of sequence information for many plants has opened the way to advanced genetic analysis in many non-model plants. Nevertheless, exploration of genetic variation on a large scale and its use as a tool for the identification of traits of interest are still rare. In this study, we combined a bulk segregation approach with our own-designed microarrays to map the pH locus that influences fruit pH in melon. Using these technologies, we identified a set of markers that are genetically linked to the pH trait. Further analysis using a set of melon cultivars demonstrated that some of these markers are tightly linked to the pH trait throughout our germplasm collection. These results validate the utility of combining microarray technology with a bulk segregation approach in mapping traits of interest in non-model plants. © 2012 Springer-Verlag Berlin Heidelberg.
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Combining bulk segregation analysis and microarrays for mapping of the pH trait in melon
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Sherman, A., Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Eshed, R., Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Harel-Beja, R., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Tzuri, G., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Portnoy, V., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Cohen, S., Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Rubinstein, M., Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Schaffer, A.A., Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Burger, J., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Katzir, N., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095 Ramat Yishay, Israel
Ophir, R., Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel
Combining bulk segregation analysis and microarrays for mapping of the pH trait in melon
The availability of sequence information for many plants has opened the way to advanced genetic analysis in many non-model plants. Nevertheless, exploration of genetic variation on a large scale and its use as a tool for the identification of traits of interest are still rare. In this study, we combined a bulk segregation approach with our own-designed microarrays to map the pH locus that influences fruit pH in melon. Using these technologies, we identified a set of markers that are genetically linked to the pH trait. Further analysis using a set of melon cultivars demonstrated that some of these markers are tightly linked to the pH trait throughout our germplasm collection. These results validate the utility of combining microarray technology with a bulk segregation approach in mapping traits of interest in non-model plants. © 2012 Springer-Verlag Berlin Heidelberg.
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