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Biochemical and transcriptomic analysis of host responses to yeast biocontrol agents of postharvest pathogens
Year:
2013
Source of publication :
Journal of Plant Pathology
Authors :
Droby, Samir
;
.
Feygenberg, Oleg
;
.
Hershkovitz, Vera
;
.
Kessler (Ben-Dayan), Clarita
;
.
Rafael, Ginat
;
.
Sela, Noa
;
.
Volume :
1012
Co-Authors:

Droby, S., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Hershkovitz, V., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Sela, N., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Raphael, G., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Kessler, C., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Feygenberg, O., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Wisniewski, M., United States Department, Agriculture (USDA-ARS) WV, United States



 

Facilitators :
From page:
671
To page:
680
(
Total pages:
10
)
Abstract:
Although the biocontrol activity of antagonistic yeasts has been demonstrated on a variety of commodities, the mode of action of these microbial biocontrol agents has not been fully elucidated. Antagonistic yeasts of postharvest pathogens have been reported to induce several biochemical defense responses in host tissues of surface-wounded fruit. The interaction of yeast cells with fruit tissue resulted in major responses of the fruit that included enhanced production of ROS, phytoalexins, fungal cell wall degrading enzymes and the formation of structural barriers such as deposition of papillae and lignin in host cell walls. We have also shown that interaction of yeast cells with fruit tissue alters the volatile profile by down-regulation or up-regulation of certain compounds. To gain a better understanding of the molecular changes taking place in fruit tissue following the application of the yeast Metschnikowia fructicola, microarray analysis was performed on grapefruit surface wounds. The data indicated that 1007 putative unigenes showed significant expression changes following wounding and yeast application compared to wounded controls. Microarray results of select genes were validated by RT-qPCR. The data indicated that yeast application induced expression of Rbo, MAPK and MAPKK, G-proteins, CHI, PAL, CHS, 4CL. In contrast, three genes POD, SOD and CAT were down- regulated in grapefruit peel tissue treated with the yeast cells. Suppression was correlated with significantly higher levels of hydrogen peroxide, superoxide anion and hydroxyl radical production in yeast-treated surface wounds. This study provides the first global picture of gene expression changes in grapefruit in response to yeast antagonists.
Note:
Related Files :
grapefruits
Metschnikowia fructicola
ROS
Volatile compounds
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
article
;
.
Language:
English
Editors' remarks:
ID:
26372
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:22
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Scientific Publication
Biochemical and transcriptomic analysis of host responses to yeast biocontrol agents of postharvest pathogens
1012

Droby, S., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Hershkovitz, V., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Sela, N., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Raphael, G., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Kessler, C., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Feygenberg, O., Institute of Postharvest and Food Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Wisniewski, M., United States Department, Agriculture (USDA-ARS) WV, United States



 

Biochemical and transcriptomic analysis of host responses to yeast biocontrol agents of postharvest pathogens
Although the biocontrol activity of antagonistic yeasts has been demonstrated on a variety of commodities, the mode of action of these microbial biocontrol agents has not been fully elucidated. Antagonistic yeasts of postharvest pathogens have been reported to induce several biochemical defense responses in host tissues of surface-wounded fruit. The interaction of yeast cells with fruit tissue resulted in major responses of the fruit that included enhanced production of ROS, phytoalexins, fungal cell wall degrading enzymes and the formation of structural barriers such as deposition of papillae and lignin in host cell walls. We have also shown that interaction of yeast cells with fruit tissue alters the volatile profile by down-regulation or up-regulation of certain compounds. To gain a better understanding of the molecular changes taking place in fruit tissue following the application of the yeast Metschnikowia fructicola, microarray analysis was performed on grapefruit surface wounds. The data indicated that 1007 putative unigenes showed significant expression changes following wounding and yeast application compared to wounded controls. Microarray results of select genes were validated by RT-qPCR. The data indicated that yeast application induced expression of Rbo, MAPK and MAPKK, G-proteins, CHI, PAL, CHS, 4CL. In contrast, three genes POD, SOD and CAT were down- regulated in grapefruit peel tissue treated with the yeast cells. Suppression was correlated with significantly higher levels of hydrogen peroxide, superoxide anion and hydroxyl radical production in yeast-treated surface wounds. This study provides the first global picture of gene expression changes in grapefruit in response to yeast antagonists.
Scientific Publication
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