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Regulation of calbindin mRNA and calbindin turnover in intestine and shell gland of the chicken
Year:
1992
Authors :
Bär, Arie
;
.
Hurvitz, Shmuel (Animal science)
;
.
Striem, Sarina
;
.
Talpaz, Hovav
;
.
Wax, Eliyahu
;
.
Volume :
262
Co-Authors:
Bar, A., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Striem, S., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Vax, E., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Talpaz, H., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Hurwitz, S., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Facilitators :
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0
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Total pages:
1
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Abstract:
A synthetic oligonucleotide was used as a probe for measurement of calbindin mRNA in the shell gland and intestine of chickens. The half time of calbindin mRNA in the duodenum and shell gland was estimated at 2 and 3.6 h and that of calbindin at 13.9 and 32.6 h, respectively. The formation rates of calbindin mRNA were 0.37 and 0.17 pmol · h-1 · g-1 and the rate of calbindin formation was 0.099 and 0.031 μg · pmol mRNA-1 · h-1 in the duodenum and shell gland, respectively. In the shell gland, calbindin mRNA and calbindin appeared at the time of sexual maturation during calcification of the first egg shell. Calbindin mRNA fluctuated markedly during the daily egg cycle, in close temporal association with egg shell calcification. When Ca2+ deposition was eliminated by expulsion of the ovum, the rise in calbindin mRNA was prevented. An indirect suppression of Ca2+ deposition by administration of the carbonic anhydrase inhibitor acetazolamide also resulted in a decrease in calbindin mRNA. The results are consistent with a possible role of Ca2+ flux in the regulation of calbindin mRNA appearance in the shell gland of chickens.
Note:
Related Files :
Acetazolamide
Animal
animal experiment
Chickens
Female
gene expression
messenger rna synthesis
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DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26412
Last updated date:
21/08/2022 07:45
Creation date:
17/04/2018 00:22
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Scientific Publication
Regulation of calbindin mRNA and calbindin turnover in intestine and shell gland of the chicken
262
Bar, A., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Striem, S., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Vax, E., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Talpaz, H., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Hurwitz, S., Volcani Center, Institute of Animal Science, ARO, POB 6, Bet Dagan 50250, Israel
Regulation of calbindin mRNA and calbindin turnover in intestine and shell gland of the chicken
A synthetic oligonucleotide was used as a probe for measurement of calbindin mRNA in the shell gland and intestine of chickens. The half time of calbindin mRNA in the duodenum and shell gland was estimated at 2 and 3.6 h and that of calbindin at 13.9 and 32.6 h, respectively. The formation rates of calbindin mRNA were 0.37 and 0.17 pmol · h-1 · g-1 and the rate of calbindin formation was 0.099 and 0.031 μg · pmol mRNA-1 · h-1 in the duodenum and shell gland, respectively. In the shell gland, calbindin mRNA and calbindin appeared at the time of sexual maturation during calcification of the first egg shell. Calbindin mRNA fluctuated markedly during the daily egg cycle, in close temporal association with egg shell calcification. When Ca2+ deposition was eliminated by expulsion of the ovum, the rise in calbindin mRNA was prevented. An indirect suppression of Ca2+ deposition by administration of the carbonic anhydrase inhibitor acetazolamide also resulted in a decrease in calbindin mRNA. The results are consistent with a possible role of Ca2+ flux in the regulation of calbindin mRNA appearance in the shell gland of chickens.
Scientific Publication
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