אסיף מאגר המחקר החקלאי

Short- and long-term mechanisms of tau regulation in PC12 cells

Year:

1995

Source of publication :

Authors :

Volume :

108

Co-Authors:

Sadot, E., Department of Neurobiology, The Weizmann Institute of Science, PO Box 26, 76100 Rehovot, Israel
Barg, J., Department of Neurobiology, The Weizmann Institute of Science, PO Box 26, 76100 Rehovot, Israel
Rasouly, D., Department of Neurobiology, The Weizmann Institute of Science, PO Box 26, 76100 Rehovot, Israel
Lazarovici, P., Department of Neurobiology, The Weizmann Institute of Science, PO Box 26, 76100 Rehovot, Israel
Ginzburg, I., Department of Neurobiology, The Weizmann Institute of Science, PO Box 26, 76100 Rehovot, Israel

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Abstract:

Induction by nerve growth factor of neurite outgrowth in PC12 cells is transcription-dependent and is associated with the accumulation of tau protein. It was recently shown that short-term treatment with staurosporine, a protein kinase alkaloid inhibitor, induced an elevation of tau protein levels and outgrowth of stable neurites. In this study, we analyzed the mechanism(s) by which nerve growth factor and staurosporine exert their effects on tau levels. We demonstrate that nerve growth factor affects tau mRNA stability, thus contributing to the observed increase in tau mRNA levels. On the other hand, tau mRNA levels were not affected by the treatment with staurosporine. We also demonstrate that the phosphorylation of tau protein was reduced after treatment of PC12 cells with nerve growth factor or staurosporine, as shown by immunoblot analysis using specific antibodies and alkaline phosphatase treatment. Thus, regulation of tau levels by nerve growth factor appears to be mediated by transcriptional, posttranscriptional and posttranslational steps, whereas the effect of staurosporine on tau levels may be attributed to its effect on the state of phosphorylation of the protein.

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