Co-Authors:
Mazor, L., Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Perl, M., Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Negbi, M., Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Abstract:
During the imbibition of seeds in polyethyleneglycol (PEG), increasing amounts of ATP accumulated up to 24 h. Similar amounts accumulated in the seeds during 4-5 h of imbibition in water. Radioactive amino acids were increasingly incorporated in the acid-insoluble fraction up to 24 h imbibition in PEG, as well as in water, after which a sharp decrease occurred up to 5 d of imbibition. If seeds were imbibed in PEG or water in the presence of radioactive acetate, water-insoluble radioactivity increased linearly in seeds during 5 d of imbibition. The amounts of incorporated amino acids or acetate were about double in PEG-imbibed as compared with in water-imbibed seed. The incorporation of AMP into the acid-insoluble fraction in seeds imbibed in PEG in the presence of radioactive AMP levelled off after 24 h followed by a sharp decrease of up to 10% of the peak 5 d after the start of imbibition. In water-imbibed seeds the incorporation of AMP continued to increase during at least 5 d of imbibition. During redrying of PEG-treated seeds (24 h), at least 80% of the accumulated ATP decreased during 18 d. The total radioactive amino acids and nucleotide decreased during 3 d of redrying by 20% and 60%, respectively. At that time, the acid-insoluble incorporates increased by 20% and 50%, respectively. Some of the AMP was released as CO 2. © 1984 Oxford University Press.