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Promoters of Mycoplasma capricolum ribosomal RNA operons: Identical activities but different regulation in homologous and heterologous cells
Year:
1988
Source of publication :
Nucleic Acids Research
Authors :
Gafny, Ron
;
.
Volume :
16
Co-Authors:
Gafny, R., Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Hyman, H.C., Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Razin, S., Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Glaser, G., Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Facilitators :
From page:
61
To page:
76
(
Total pages:
16
)
Abstract:
The 5' region of the rRNA operon, rrnA, of M. capricolum was cloned. Sequence analysis revealed two tRNA genes, tRNAleu and tRNAlys, upstream to the promoter of the rRNA operon. The in vivo transcription start sites of the rRNA operon and of the tRNA genes were mapped. The same promoters used by M. capricolum RNA polymerase are also recognized by E. coli RNA polymerase both in vivo and in vitro. We find that high levels of ppGpp in E. coll, resulting from amino acid starvation or from spoT mutation, activate rather than repress the transcription of the mycoplasma rrnA operon. © 1988 IRL Press Limited, Oxford, England.
Note:
Related Files :
Base Sequence
Genetics
molecular genetics
mycoplasma
regulator gene
RNA, Ribosomal
RNA, Transfer, Lys
Show More
Related Content
More details
DOI :
10.1093/nar/16.1.61
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26476
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:23
Scientific Publication
Promoters of Mycoplasma capricolum ribosomal RNA operons: Identical activities but different regulation in homologous and heterologous cells
16
Gafny, R., Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Hyman, H.C., Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Razin, S., Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Glaser, G., Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Promoters of Mycoplasma capricolum ribosomal RNA operons: Identical activities but different regulation in homologous and heterologous cells
The 5' region of the rRNA operon, rrnA, of M. capricolum was cloned. Sequence analysis revealed two tRNA genes, tRNAleu and tRNAlys, upstream to the promoter of the rRNA operon. The in vivo transcription start sites of the rRNA operon and of the tRNA genes were mapped. The same promoters used by M. capricolum RNA polymerase are also recognized by E. coli RNA polymerase both in vivo and in vitro. We find that high levels of ppGpp in E. coll, resulting from amino acid starvation or from spoT mutation, activate rather than repress the transcription of the mycoplasma rrnA operon. © 1988 IRL Press Limited, Oxford, England.
Scientific Publication
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