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Multistage disruption of protoplasts by dikegulac
Year:
1980
Source of publication :
Planta
Authors :
Zilkah, Shmuel
;
.
Volume :
147
Co-Authors:
Zilkah, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
274
To page:
276
(
Total pages:
3
)
Abstract:
Dikegulac (2,3:4,6 di-o-isopropylidine-2-keto-I-gulonate) is a growth regulator used to differentially kill terminal apices, and it analogously inhibits basic metabolic functions in dividing cells, but not stationary cells, in suspension culture. This report demonstrates an analogous situation in isolated tobacco protoplasts. At the lowest concentrations, dikegulac partially suppresses division of the protoplasts. Higher concentrations are required to produce visual cytoplasmic damage to the protoplasts, which probably first occurs at the level of the plasmalemma, as the vacuoles can be released intact. Later, tonoplast disruption occurs. © 1980 Springer-Verlag.
Note:
Related Files :
Cell Division
dikegulac
Lysis
Nicotiana
Plasmalemma
Protoplasts
Tonoplast
Show More
Related Content
More details
DOI :
10.1007/BF00379832
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26478
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:23
Scientific Publication
Multistage disruption of protoplasts by dikegulac
147
Zilkah, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Multistage disruption of protoplasts by dikegulac
Dikegulac (2,3:4,6 di-o-isopropylidine-2-keto-I-gulonate) is a growth regulator used to differentially kill terminal apices, and it analogously inhibits basic metabolic functions in dividing cells, but not stationary cells, in suspension culture. This report demonstrates an analogous situation in isolated tobacco protoplasts. At the lowest concentrations, dikegulac partially suppresses division of the protoplasts. Higher concentrations are required to produce visual cytoplasmic damage to the protoplasts, which probably first occurs at the level of the plasmalemma, as the vacuoles can be released intact. Later, tonoplast disruption occurs. © 1980 Springer-Verlag.
Scientific Publication
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