אסיף מאגר המחקר החקלאי

Antioxidative potential of folate producing probiotic Lactobacillus helveticus CD6

Year:

2013

Source of publication :

Authors :

Volume :

50

Co-Authors:

Ahire, J.J., Department of Microbiology, School of Life Sciences, North Maharashtra University, Umavi Nagar Jalgaon Post Box 80, 425 001 Maharashtra, India
Mokashe, N.U., Department of Microbiology, School of Life Sciences, North Maharashtra University, Umavi Nagar Jalgaon Post Box 80, 425 001 Maharashtra, India
Patil, H.J., Department of Microbiology, School of Life Sciences, North Maharashtra University, Umavi Nagar Jalgaon Post Box 80, 425 001 Maharashtra, India, National Bureau of Agriculturally Important Microorganisms, Mau 275101, India
Chaudhari, B.L., Department of Microbiology, School of Life Sciences, North Maharashtra University, Umavi Nagar Jalgaon Post Box 80, 425 001 Maharashtra, India

Facilitators :

Link :

Abstract:

Folate producing Lactobacillus sp. CD6 isolated from fermented milk showed 98% similarity with Lactobacillus helveticus based on 16S rRNA gene sequence analysis. It was found to produce a folic acid derivative 5-methyl tetrahydrofolate (5-MeTHF). The intracellular cell-free extract of strain demonstrated antioxidative activity with the inhibition rate of ascorbate autoxidation in the range of 27.5%±3.7%. It showed highest metal ion chelation ability for Fe2+ (0.26±0.06 ppm) as compared to Cu2+. The DPPH (α,α-Diphenyl-β-Picrylhydrazyl) radical scavenging activity for intact cells were found to be 24.7%±10.9% proved its antioxidative potential. Furthermore, it demonstrated 14.89% inhibition of epinephrine autoxidation, 20.9±1.8 μg cysteine equivalent reducing activity and 20.8%±0.9% hydroxyl radical scavenging effect. The strain was evaluated for probiotic properties as per WHO and FAO guidelines. It showed 90.61% survival at highly acidic condition (pH 2.0), 90.66% viability in presence of synthetic gastric juice and 68% survivability at 0.5% bile concentration for 24 h. It was susceptible to many antibiotics which reduces the prospect to offer resistance determinants to other organisms if administered in the form of probiotic preparations. It showed in vitro mucus binding and antimicrobial activity against enteric pathogens like Salmonella typhimurium (NCIM 2501), Streptococcus pyogenes (NCIM 2608), and Staphylococcus aureus (NCIM 5021) and moreover it showed non- hemolytic activity on sheep blood agar. © 2011 Association of Food Scientists & Technologists (India).

Note:

Related Files :

Related Content

More details

You may also be interested in