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The effect of parathyroid hormone and atrial natriuretic peptide on cyclic nucleotides production and proliferation of avian epiphyseal growth plate chondroprogenitor cells
Year:
1988
Source of publication :
Endocrinology
Authors :
Hurvitz, Shmuel (Animal science)
;
.
Pines, Mark
;
.
Volume :
123
Co-Authors:
Pines, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Hurwitz, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
360
To page:
365
(
Total pages:
6
)
Abstract:
Cells derived from avian tibia epiphyseal growth plate were cultured in vitro. The cells which exhibited a polygonal phenotype and are termed chondroprogenitor cells, developed in culture as a monolayer with a doubling time of 40†48 h in 5% fetal calf serum. Production of cAMP by the chondroprogenitor cells was stimulated by human (1†34) and bovine native (1†84) PTH. The effect of PTH on cAMP production could be blocked by the (3†34) PTH analog, suggesting interaction with specific receptors. cAMP production by avian chondroprogenitor cells was also stimulated by cholera toxin, forskolin, and prostaglandin E2but not by ACTH or prostaglandin F2α. PTH, cholera toxin, and forskolin also stimulated proliferation of the chondroprogenitor cells. In contrast, neither cAMP production nor proliferation of avian skin fibroblasts was affected by PTH. Human (1†28) and rat (5†28) atrial natriuretic peptide stimulated cGMP production by avian chondroprogentior cells and also by skin fibroblasts. Atrial natriuretic peptide inhibited the basal and PTH-stimulated [31H]thymidine incorporation into DNA of chondroprogenitor cells, but did not affect avian skin fibroblast proliferation. These results suggest that the proliferation of avian epiphyseal growth plate chondroprogenitor cells is modulated by opposing mechanisms induced by PTH and ANP, probably mediated by cAMP and cGMP, respectively. © 1988 by The Endocrine Society.
Note:
Related Files :
1-Methyl-3-isobutylxanthine
Animal
animal cell
Cyclic AMP
Growth plate
Histology
Peptide Fragments
tissue culture
Show More
Related Content
More details
DOI :
10.1210/endo-123-1-360
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
26497
Last updated date:
21/08/2022 07:45
Creation date:
17/04/2018 00:23
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Scientific Publication
The effect of parathyroid hormone and atrial natriuretic peptide on cyclic nucleotides production and proliferation of avian epiphyseal growth plate chondroprogenitor cells
123
Pines, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Hurwitz, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
The effect of parathyroid hormone and atrial natriuretic peptide on cyclic nucleotides production and proliferation of avian epiphyseal growth plate chondroprogenitor cells
Cells derived from avian tibia epiphyseal growth plate were cultured in vitro. The cells which exhibited a polygonal phenotype and are termed chondroprogenitor cells, developed in culture as a monolayer with a doubling time of 40†48 h in 5% fetal calf serum. Production of cAMP by the chondroprogenitor cells was stimulated by human (1†34) and bovine native (1†84) PTH. The effect of PTH on cAMP production could be blocked by the (3†34) PTH analog, suggesting interaction with specific receptors. cAMP production by avian chondroprogenitor cells was also stimulated by cholera toxin, forskolin, and prostaglandin E2but not by ACTH or prostaglandin F2α. PTH, cholera toxin, and forskolin also stimulated proliferation of the chondroprogenitor cells. In contrast, neither cAMP production nor proliferation of avian skin fibroblasts was affected by PTH. Human (1†28) and rat (5†28) atrial natriuretic peptide stimulated cGMP production by avian chondroprogentior cells and also by skin fibroblasts. Atrial natriuretic peptide inhibited the basal and PTH-stimulated [31H]thymidine incorporation into DNA of chondroprogenitor cells, but did not affect avian skin fibroblast proliferation. These results suggest that the proliferation of avian epiphyseal growth plate chondroprogenitor cells is modulated by opposing mechanisms induced by PTH and ANP, probably mediated by cAMP and cGMP, respectively. © 1988 by The Endocrine Society.
Scientific Publication
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