Theoretical and Applied Genetics
Petrova, M., Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria
Vulkova, Z., Institute of Genetics, 1113 Sofia, Bulgaria
Gorinova, N., Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria
Izhar, S., ARO, Institute of Field and Garden Crops, Bet-Dagan, Israel
Firon, N., ARO, Institute of Field and Garden Crops, Bet-Dagan, Israel
Jacquemin, J.-M., Station d'Amelioration des Plantes, 5030 Gembloux, Belgium
Atanassov, A., De Montfort University, Norman Borlaug Ctr. Plant Sci. Res., Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria
Stoeva, P., De Montfort University, Norman Borlaug Ctr. Plant Sci. Res., Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria
The cytoplasmic male-sterile (CMS) line CMS-pennellii (BC10P2 L. peruvianum x L. pennellii) and its complex hybrids with L. esculentum were studied. The established sterility was classified as the sporogenous type. As a result of the interaction of the genome of L. pennellii and the cytoplasm of L. peruvianum clear changes were established in the profiles of malic enzyme and esterase. Restriction fragment length polymorphism (RFLP) was detected between the mitochondrial (mt) genomes of CMS-pennellii and the cytoplasm donor, L. peruvianum, for two mtDNA probes: atpA and nad3. The established differences in the isozyme pattern and mt genomes are considered as useful markers to distinguish fertile and sterile plants. A breakthrough in the unilateral incompatibility of CMS-pennellii and the incorporation of the genome of L. esculentum on a CMS background is reported. The analysis of the complex hybrids assumes the interaction of two dominant genes - a maintainer gene from L. pennellii and a restorer gene from cultivated tomato. The hybrids produced with L. esculentum provide the basis for the development of a CMS system in cultivated tomato.
Powered by ClearMash Solutions Ltd -
Volcani treasures
About
Terms of use
Characterisation of a cytoplasmic male-sterile hybrid line between Lycopersicon peruvianum Mill. x Lycopersicon pennellii Corr. and its crosses with cultivated tomato
98
Petrova, M., Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria
Vulkova, Z., Institute of Genetics, 1113 Sofia, Bulgaria
Gorinova, N., Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria
Izhar, S., ARO, Institute of Field and Garden Crops, Bet-Dagan, Israel
Firon, N., ARO, Institute of Field and Garden Crops, Bet-Dagan, Israel
Jacquemin, J.-M., Station d'Amelioration des Plantes, 5030 Gembloux, Belgium
Atanassov, A., De Montfort University, Norman Borlaug Ctr. Plant Sci. Res., Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria
Stoeva, P., De Montfort University, Norman Borlaug Ctr. Plant Sci. Res., Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria
Characterisation of a cytoplasmic male-sterile hybrid line between Lycopersicon peruvianum Mill. x Lycopersicon pennellii Corr. and its crosses with cultivated tomato
The cytoplasmic male-sterile (CMS) line CMS-pennellii (BC10P2 L. peruvianum x L. pennellii) and its complex hybrids with L. esculentum were studied. The established sterility was classified as the sporogenous type. As a result of the interaction of the genome of L. pennellii and the cytoplasm of L. peruvianum clear changes were established in the profiles of malic enzyme and esterase. Restriction fragment length polymorphism (RFLP) was detected between the mitochondrial (mt) genomes of CMS-pennellii and the cytoplasm donor, L. peruvianum, for two mtDNA probes: atpA and nad3. The established differences in the isozyme pattern and mt genomes are considered as useful markers to distinguish fertile and sterile plants. A breakthrough in the unilateral incompatibility of CMS-pennellii and the incorporation of the genome of L. esculentum on a CMS background is reported. The analysis of the complex hybrids assumes the interaction of two dominant genes - a maintainer gene from L. pennellii and a restorer gene from cultivated tomato. The hybrids produced with L. esculentum provide the basis for the development of a CMS system in cultivated tomato.
Scientific Publication