אסיף מאגר המחקר החקלאי

Transgenic tomato plants expressing the tomato yellow leaf curl virus capsid protein are resistant to the virus (from: Nature Biotechnology)

Year:

1994

Source of publication :

Authors :

Volume :

12

Co-Authors:

Zamir, D., Dept. of Field and Vegetable Crops, Otto Warburg Ctr. Biotech. in Agric., Hebrew University of Jerusalem, P.O.BOX 12, Rehovot 76100, Israel
Navot, N., Dept. of Field and Vegetable Crops, Otto Warburg Ctr. Biotech. in Agric., Hebrew University of Jerusalem, P.O.BOX 12, Rehovot 76100, Israel
Zeidan, M., Dept. of Field and Vegetable Crops, Otto Warburg Ctr. Biotech. in Agric., Hebrew University of Jerusalem, P.O.BOX 12, Rehovot 76100, Israel
Michelson, I., Dept. of Field and Vegetable Crops, Otto Warburg Ctr. Biotech. in Agric., Hebrew University of Jerusalem, P.O.BOX 12, Rehovot 76100, Israel
Czosnek, H., Dept. of Field and Vegetable Crops, Otto Warburg Ctr. Biotech. in Agric., Hebrew University of Jerusalem, P.O.BOX 12, Rehovot 76100, Israel

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Abstract:

The tomato yellow leaf curl virus (TYLCV) gene that encodes the capsid protein (VI) was placed under transcriptional control of the cauliflower mosaic virus 35S promoter and cloned into an Agrobacterium Ti-derived plasmid and used to transform plants from an interspecific tomato hybrid, Lycopersicon esculentum X L. pennellii (F1), sensitive to the TYLCV disease. When transgenic F1 plants, expressing the VI gene, were inoculated with TYLCV using whiteflies fed on TYLCV-infected plants, they responded either as untransformed tomato or showed expression of delayed disease symptoms and recovery from the disease with increasingly more resistance upon repeated inoculation. Transformed plants that were as sensitive to inoculation as untransformed controls expressed the VI gene at the RNA level only. All the transformed plants that recovered from disease expressed the TYLCV capsid protein.

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